Biomaterial Database

Silver stain for proteins in ultrathin polyacrylamide gels: a sensitive precipitation technique. 1988

H Kling, and G Sawatzki, and W Geis

Abteilung Humangenetik, Universität Ulm, German Federal Republic.

A rapid and highly sensitive silver stain for visualization of proteins on ultrathin isoelectric focusing gels is described. This procedure is based on the specific interaction of silver and bromide ions in the presence of proteins and appears to involve a precipitation reaction. The technique requires only two reaction solutions, a silver nitrate and a potassium bromide solution. Silver consumption is very low because the silver nitrate solution is reusable. This procedure is well established for proteins separated by isoelectrofocusing in ultrathin gels.

UI	MeSH Term	Description	Entries
D011232	Chemical Precipitation	The formation of a solid in a solution as a result of a chemical reaction or the aggregation of soluble substances into complexes large enough to fall out of solution.	Precipitation, Chemical
D011506	Proteins	Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.	Gene Products, Protein,Gene Proteins,Protein,Protein Gene Products,Proteins, Gene
D004591	Electrophoresis, Polyacrylamide Gel	Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.	Polyacrylamide Gel Electrophoresis,SDS-PAGE,Sodium Dodecyl Sulfate-PAGE,Gel Electrophoresis, Polyacrylamide,SDS PAGE,Sodium Dodecyl Sulfate PAGE,Sodium Dodecyl Sulfate-PAGEs
D012834	Silver	An element with the atomic symbol Ag, atomic number 47, and atomic weight 107.87. It is a soft metal that is used medically in surgical instruments, dental prostheses, and alloys. Long-continued use of silver salts can lead to a form of poisoning known as ARGYRIA.
D013194	Staining and Labeling	The marking of biological material with a dye or other reagent for the purpose of identifying and quantitating components of tissues, cells or their extracts.	Histological Labeling,Staining,Histological Labelings,Labeling and Staining,Labeling, Histological,Labelings, Histological,Stainings