BIOMAT Database Logo BIOMAT Database Logo

Rapid isolation of total RNA from mammalian tissues. 1988

M Emmett, and B Petrack
Research Department, CIBA-GEIGY Corporation, Summit, New Jersey 07901.

A rapid procedure for the isolation of total RNA from small amounts of mammalian tissue (35 to 150 mg) is described. Tissues were homogenized in the presence of RNase inhibitors but in the absence of strong detergents. Contaminants were removed by phenol/chloroform extraction and Sephadex column chromatography. Total RNAs were precipitated with ethanol and sodium acetate. The RNAs isolated were intact and suitable for mRNA quantitation via Northern blot or slot-blot analyses. This procedure isolates total RNAs in high yield and purity, without CsCl ultracentrifugation, and is especially useful when mRNAs must be quantitated from many samples.

UI MeSH Term Description Entries
D002850 Chromatography, Gel Chromatography on non-ionic gels without regard to the mechanism of solute discrimination. Chromatography, Exclusion,Chromatography, Gel Permeation,Chromatography, Molecular Sieve,Gel Filtration,Gel Filtration Chromatography,Chromatography, Size Exclusion,Exclusion Chromatography,Gel Chromatography,Gel Permeation Chromatography,Molecular Sieve Chromatography,Chromatography, Gel Filtration,Exclusion Chromatography, Size,Filtration Chromatography, Gel,Filtration, Gel,Sieve Chromatography, Molecular,Size Exclusion Chromatography
D004586 Electrophoresis An electrochemical process in which macromolecules or colloidal particles with a net electric charge migrate in a solution under the influence of an electric current. Electrophoreses
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D012313 RNA A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached. RNA is unique among biological macromolecules in that it can encode genetic information, serve as an abundant structural component of cells, and also possesses catalytic activity. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed) RNA, Non-Polyadenylated,Ribonucleic Acid,Gene Products, RNA,Non-Polyadenylated RNA,Acid, Ribonucleic,Non Polyadenylated RNA,RNA Gene Products,RNA, Non Polyadenylated
D013997 Time Factors Elements of limited time intervals, contributing to particular results or situations. Time Series,Factor, Time,Time Factor
D051381 Rats The common name for the genus Rattus. Rattus,Rats, Laboratory,Rats, Norway,Rattus norvegicus,Laboratory Rat,Laboratory Rats,Norway Rat,Norway Rats,Rat,Rat, Laboratory,Rat, Norway,norvegicus, Rattus

Related Publications

M Emmett, and B Petrack
Total RNA Isolation from Planarian Tissues.
January 2018, Methods in molecular biology (Clifton, N.J.),
M Emmett, and B Petrack
Rapid isolation of total RNA from small samples of hypocellular, dense connective tissues.
June 1997, BioTechniques,
M Emmett, and B Petrack
Purification of Total RNA from Mammalian Cells and Tissues.
January 2020, Cold Spring Harbor protocols,
M Emmett, and B Petrack
RNA extraction from mammalian tissues.
January 2007, Methods in molecular biology (Clifton, N.J.),
M Emmett, and B Petrack
RNA isolation from mammalian samples.
July 2013, Current protocols in molecular biology,
M Emmett, and B Petrack
Rapid isolation of total acidic proteins from chromatin of various chick tissues.
September 1973, Biochimica et biophysica acta,
M Emmett, and B Petrack
Improved proteinase K digestion for the rapid isolation of mRNA from mammalian tissues.
October 1993, BioTechniques,
M Emmett, and B Petrack
Rapid isolation of high-quality total RNA from taxus and ginkgo.
August 2004, Preparative biochemistry & biotechnology,
M Emmett, and B Petrack
Isolation of total RNA from tissues or cell lines. Visualization in gel.
January 1998, Methods in molecular biology (Clifton, N.J.),
M Emmett, and B Petrack
A new rapid method for selective extraction of RNA from fixed mammalian tissues.
January 1978, Folia histochemica et cytochemica,
Copied contents to your clipboard!