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Although there have been several reports pertaining to the existence of the cGMP-gated channel in the disk membrane of rod photoreceptors, its density there relative to that of the photoreceptor plasma membrane is unknown. Using immunoblotting, immunohistochemical, and reconstitution techniques on purified disk and plasma membrane preparations, we found that the density of channels in the plasma membrane was at least 50-fold higher than that of the disk membrane. Purification of membrane fractions without prior digestion of cytoskeletal components by mild trypsinization was found to increase the amount of channel protein present in disk membrane preparations. We propose that the presence of the channel protein in rod disk membrane preparations is an artifact arising from fusion of plasma membrane components during permeabilization of the photoreceptor cell.
N J Cook, and
L L Molday, and
D Reid, and
U B Kaupp, and
R S Molday
May 1998,
Vision research,
N J Cook, and
L L Molday, and
D Reid, and
U B Kaupp, and
R S Molday
December 1989,
The Journal of biological chemistry,
N J Cook, and
L L Molday, and
D Reid, and
U B Kaupp, and
R S Molday
April 1995,
The Journal of physiology,
N J Cook, and
L L Molday, and
D Reid, and
U B Kaupp, and
R S Molday
February 2005,
Biochemistry,
N J Cook, and
L L Molday, and
D Reid, and
U B Kaupp, and
R S Molday
January 1988,
Cold Spring Harbor symposia on quantitative biology,
N J Cook, and
L L Molday, and
D Reid, and
U B Kaupp, and
R S Molday
January 2002,
Advances in experimental medicine and biology,
N J Cook, and
L L Molday, and
D Reid, and
U B Kaupp, and
R S Molday
May 2000,
The Journal of biological chemistry,
N J Cook, and
L L Molday, and
D Reid, and
U B Kaupp, and
R S Molday
January 2001,
Visual neuroscience,
N J Cook, and
L L Molday, and
D Reid, and
U B Kaupp, and
R S Molday
December 1996,
Biophysical journal,
N J Cook, and
L L Molday, and
D Reid, and
U B Kaupp, and
R S Molday
February 1997,
Experimental eye research,
