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On the existence of a Na+-Ca2+ exchange system in the cell membrane of vascular smooth muscle. 1988

M A Matlib
Department of Pharmacology and Cell Biophysics, University of Cincinnati College of Medicine, OH 45267-0575.

Ca2+ uptake was studied in Na+-loaded cell membrane vesicles isolated from blood vessels of several species. A rapid uptake of Ca2+ was observed in an isotonic KCl medium when there was an outwardly directed Na+ concentration gradient created across the membrane. Elimination of the Na+ concentration gradient by including Na+ or the sodium ionophore monensin in the assay medium resulted in drastic reduction of Ca2+ uptake in the vesicles. This Ca2+ uptake was found to be specific for Na+ since other monovalent cations did not substitute Na+. Employing this approach, a Na+-Ca2+ exchange system was demonstrated in dog mesenteric artery, rat mesenteric artery, rat aorta, and rabbit aorta. The apparent Km for free Ca2+ of this process in these smooth muscles varies from 1.61 to 2.72 microM and the apparent maximum velocity varies from 7 to 16 nmol/min/mg protein. The results of the study with isolated membrane vesicles indicate the existence of a Na+-Ca2+ exchange in the cell membrane of vascular smooth muscle.

UI MeSH Term Description Entries
D007474 Ion Exchange Reversible chemical reaction between a solid, often one of the ION EXCHANGE RESINS, and a fluid whereby ions may be exchanged from one substance to another. This technique is used in water purification, in research, and in industry. Exchange, Ion
D008297 Male Males
D009131 Muscle, Smooth, Vascular The nonstriated involuntary muscle tissue of blood vessels. Vascular Smooth Muscle,Muscle, Vascular Smooth,Muscles, Vascular Smooth,Smooth Muscle, Vascular,Smooth Muscles, Vascular,Vascular Smooth Muscles
D011919 Rats, Inbred Strains Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations or by parent x offspring matings carried out with certain restrictions. This also includes animals with a long history of closed colony breeding. August Rats,Inbred Rat Strains,Inbred Strain of Rat,Inbred Strain of Rats,Inbred Strains of Rats,Rat, Inbred Strain,August Rat,Inbred Rat Strain,Inbred Strain Rat,Inbred Strain Rats,Inbred Strains Rat,Inbred Strains Rats,Rat Inbred Strain,Rat Inbred Strains,Rat Strain, Inbred,Rat Strains, Inbred,Rat, August,Rat, Inbred Strains,Rats Inbred Strain,Rats Inbred Strains,Rats, August,Rats, Inbred Strain,Strain Rat, Inbred,Strain Rats, Inbred,Strain, Inbred Rat,Strains, Inbred Rat
D002118 Calcium A basic element found in nearly all tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes. Coagulation Factor IV,Factor IV,Blood Coagulation Factor IV,Calcium-40,Calcium 40,Factor IV, Coagulation
D002462 Cell Membrane The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells. Plasma Membrane,Cytoplasmic Membrane,Cell Membranes,Cytoplasmic Membranes,Membrane, Cell,Membrane, Cytoplasmic,Membrane, Plasma,Membranes, Cell,Membranes, Cytoplasmic,Membranes, Plasma,Plasma Membranes
D004285 Dogs The domestic dog, Canis familiaris, comprising about 400 breeds, of the carnivore family CANIDAE. They are worldwide in distribution and live in association with people. (Walker's Mammals of the World, 5th ed, p1065) Canis familiaris,Dog
D005260 Female Females
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D012508 Sarcolemma The excitable plasma membrane of a muscle cell. (Glick, Glossary of Biochemistry and Molecular Biology, 1990) Sarcolemmas

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