-transparent.png){kind=logo}
The effects of ionophores, which can carry alkali metal cations, on platelet aggregation were examined. At an alkaline extracellular pH, alkali metal cation/H+ exchanger nigericin accelerated aggregation in K+-enriched medium, whereas it rather inhibited aggregation in Na+-enriched medium, even though the intracellular pH was only slightly alkaline. The inhibitory effect of Na+ on platelet aggregation was more clearly shown with the alkali metal cation exchanger gramicidin D. The ionophore had no effect or a slightly accelerative effect on aggregation in K+-enriched medium, whereas it significantly inhibited aggregation induced by thrombin, ADP and platelet activating factor in Na+-enriched medium. Fluorescence studies on fura-2-labeled platelets revealed that in Na+-enriched medium gramicidin D inhibited agonist-induced Ca2+ mobilization both in the presence and absence of extracellular Ca2+. These results suggest that the intracellular Na+ inhibits platelet aggregation by inhibiting Ca2+ mobilization.
S Kitagawa, and
K Usui, and
F Kametani
November 2004,
Life sciences,
S Kitagawa, and
K Usui, and
F Kametani
August 1998,
Anesthesiology,
S Kitagawa, and
K Usui, and
F Kametani
October 1997,
American journal of hypertension,
S Kitagawa, and
K Usui, and
F Kametani
March 1991,
The Japanese journal of psychiatry and neurology,
S Kitagawa, and
K Usui, and
F Kametani
May 1991,
[Rinsho ketsueki] The Japanese journal of clinical hematology,
S Kitagawa, and
K Usui, and
F Kametani
December 1992,
Journal of neurochemistry,
S Kitagawa, and
K Usui, and
F Kametani
March 1989,
Biochimica et biophysica acta,
S Kitagawa, and
K Usui, and
F Kametani
January 1988,
Ukrainskii biokhimicheskii zhurnal (1978),
S Kitagawa, and
K Usui, and
F Kametani
April 1988,
Thrombosis research,
S Kitagawa, and
K Usui, and
F Kametani
October 1992,
American journal of hypertension,
