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BACKGROUND Storing pleural fluid samples for research purposes is a common practice, but whether adenosine deaminase (ADA), an enzyme used for the diagnosis of tuberculous pleuritis, is stable over long periods of time is unknown. METHODS We evaluated the stability of pleural ADA concentrations in 223 samples frozen at -800C as compared to values obtained immediately following the initial thoracentesis. Sample storage time ranged from several months to slightly more than 10 years. RESULTS ADA activity was stable for up to 2.6 years. Afterwards, it decreased 6 to 8 U/L, enough to drop 2 (3.3%) tuberculous patients below the diagnostic ADA cutoff. CONCLUSIONS As far as ADA enzymatic activity is concerned, pleural fluid samples are viable for extended periods of time. However, some caution in interpreting results from specimens stored for > 2.6 years is prudent.
S Bielsa, and
A Esquerda, and
R M Palma, and
A Criado, and
J M Porcel
July 2006,
Respirology (Carlton, Vic.),
S Bielsa, and
A Esquerda, and
R M Palma, and
A Criado, and
J M Porcel
February 1987,
The Journal of the Association of Physicians of India,
S Bielsa, and
A Esquerda, and
R M Palma, and
A Criado, and
J M Porcel
January 2002,
Problemy tuberkuleza,
S Bielsa, and
A Esquerda, and
R M Palma, and
A Criado, and
J M Porcel
April 1990,
Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases,
S Bielsa, and
A Esquerda, and
R M Palma, and
A Criado, and
J M Porcel
January 1992,
The Indian journal of chest diseases & allied sciences,
S Bielsa, and
A Esquerda, and
R M Palma, and
A Criado, and
J M Porcel
March 2004,
Clinica chimica acta; international journal of clinical chemistry,
S Bielsa, and
A Esquerda, and
R M Palma, and
A Criado, and
J M Porcel
September 1986,
Chest,
S Bielsa, and
A Esquerda, and
R M Palma, and
A Criado, and
J M Porcel
January 2005,
FEBS letters,
S Bielsa, and
A Esquerda, and
R M Palma, and
A Criado, and
J M Porcel
June 1986,
The Journal of the Association of Physicians of India,
S Bielsa, and
A Esquerda, and
R M Palma, and
A Criado, and
J M Porcel
June 2001,
The Southeast Asian journal of tropical medicine and public health,
