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We have analyzed the ability of human gamma+/delta+ T cells to recognize a nominal antigen in association with MHC molecules. A TT-specific T cell line with approximately 40% gamma+/delta+ T cells was established from a hyperimmunized donor, D.F., by stimulation with antigen and autologous APC. Three DF-derived gamma+/delta+ clones were CD8+ as determined by immunofluorescence staining, and by Southern and Northern blotting with probes detecting delta chain rearrangement and delta and gamma chain transcripts, respectively. The gamma+/delta+ clones responded to stimulation with TT, but not TNP-BSA, and autologous APC by proliferation and IFN-gamma production. No proliferation or IFN-gamma production was detected when TT-specific T cell clones were stimulated with either TT or autologous APC only. The response to TT was enhanced by addition of exogenous IL-2. The use of allogeneic APC from 19 donors sharing one HLA-determinant with the autologous donor D.F., showed that the gamma+/delta+ T cells responded to TT with HLA-DR4-related restriction as measured by proliferation and IFN-gamma production. These results demonstrate that gamma/delta receptors can recognize non-MHC-encoded foreign antigen in a self-MHC-restricted fashion.
D Kozbor, and
G Trinchieri, and
D S Monos, and
M Isobe, and
G Russo, and
J A Haney, and
C Zmijewski, and
C M Croce
September 1990,
Research in immunology,
D Kozbor, and
G Trinchieri, and
D S Monos, and
M Isobe, and
G Russo, and
J A Haney, and
C Zmijewski, and
C M Croce
January 1996,
The cancer journal from Scientific American,
D Kozbor, and
G Trinchieri, and
D S Monos, and
M Isobe, and
G Russo, and
J A Haney, and
C Zmijewski, and
C M Croce
January 1990,
Annali dell'Istituto superiore di sanita,
D Kozbor, and
G Trinchieri, and
D S Monos, and
M Isobe, and
G Russo, and
J A Haney, and
C Zmijewski, and
C M Croce
March 1992,
The Journal of investigative dermatology,
D Kozbor, and
G Trinchieri, and
D S Monos, and
M Isobe, and
G Russo, and
J A Haney, and
C Zmijewski, and
C M Croce
January 1995,
Cancer immunology, immunotherapy : CII,
D Kozbor, and
G Trinchieri, and
D S Monos, and
M Isobe, and
G Russo, and
J A Haney, and
C Zmijewski, and
C M Croce
January 1989,
International journal of cancer. Supplement = Journal international du cancer. Supplement,
D Kozbor, and
G Trinchieri, and
D S Monos, and
M Isobe, and
G Russo, and
J A Haney, and
C Zmijewski, and
C M Croce
February 1990,
[Rinsho ketsueki] The Japanese journal of clinical hematology,
D Kozbor, and
G Trinchieri, and
D S Monos, and
M Isobe, and
G Russo, and
J A Haney, and
C Zmijewski, and
C M Croce
January 1993,
International archives of allergy and immunology,
D Kozbor, and
G Trinchieri, and
D S Monos, and
M Isobe, and
G Russo, and
J A Haney, and
C Zmijewski, and
C M Croce
January 1992,
Biotherapy (Dordrecht, Netherlands),
D Kozbor, and
G Trinchieri, and
D S Monos, and
M Isobe, and
G Russo, and
J A Haney, and
C Zmijewski, and
C M Croce
October 1992,
Cancer,
