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In order to improve transformation efficiency of dehydroepiandrosterone (DHEA) into 3beta,7alpha,15alpha-trihydroxy-5-androsten-17-one (7alpha,15alpha-diOH-DHEA) by Gibberella intermedia CA3-1, we investigated the strains breeding and their conversion process optimization. G. intermedia CA3-1 strains were treated with 0.12 mg/mL 1-methyl-3-nitro-1-nitroso-guanidin (NTG) for 30 min and chosen by 350 micromol/L minimum inhibitory concentration ketoconazole resistance marker. The high production strain named M-10 with a good genetic stability was selected and the product molar yield achieved to 70.2%, which was 20% higher than that of original strain. Under the improved conversion process with the DHEA concentration of 5 g/L, the product molar yield of the mutant M-10 reached 75.6%, which was improved by 31.3% than that of original strain.
Hui Li, and
Mingjie Zhang, and
Xiaomei Zhang, and
Heng Li, and
Jinsong Shi, and
Zhenghong Xu
October 1975,
Journal of steroid biochemistry,
Hui Li, and
Mingjie Zhang, and
Xiaomei Zhang, and
Heng Li, and
Jinsong Shi, and
Zhenghong Xu
December 2009,
Bioorganic & medicinal chemistry letters,
Hui Li, and
Mingjie Zhang, and
Xiaomei Zhang, and
Heng Li, and
Jinsong Shi, and
Zhenghong Xu
July 2017,
Sheng wu gong cheng xue bao = Chinese journal of biotechnology,
Hui Li, and
Mingjie Zhang, and
Xiaomei Zhang, and
Heng Li, and
Jinsong Shi, and
Zhenghong Xu
February 2009,
Steroids,
Hui Li, and
Mingjie Zhang, and
Xiaomei Zhang, and
Heng Li, and
Jinsong Shi, and
Zhenghong Xu
December 1977,
Steroids,
Hui Li, and
Mingjie Zhang, and
Xiaomei Zhang, and
Heng Li, and
Jinsong Shi, and
Zhenghong Xu
January 1977,
Steroids,
Hui Li, and
Mingjie Zhang, and
Xiaomei Zhang, and
Heng Li, and
Jinsong Shi, and
Zhenghong Xu
February 1956,
The Journal of biological chemistry,
Hui Li, and
Mingjie Zhang, and
Xiaomei Zhang, and
Heng Li, and
Jinsong Shi, and
Zhenghong Xu
December 1963,
The Journal of clinical endocrinology and metabolism,
Hui Li, and
Mingjie Zhang, and
Xiaomei Zhang, and
Heng Li, and
Jinsong Shi, and
Zhenghong Xu
April 1987,
Journal of biochemistry,
Hui Li, and
Mingjie Zhang, and
Xiaomei Zhang, and
Heng Li, and
Jinsong Shi, and
Zhenghong Xu
January 1968,
Journal of the Chemical Society. Perkin transactions 1,
