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[Determination of lactate dehydrogenase activity using Warburg's optic test]. 1989

V I Skorniakov, and S V Skvortsov, and L A Kozhemiakin

A method for measuring the total lactate dehydrogenase activity has been developed on the basis of Warburg's optic test with a photoelectrocolorimeter, making use of lactate as a substrate. The results are calculated from the mean molar coefficient of light absorption. The coefficient of variations of the method is 7.7%. The results obtained with the use of a spectrophotometer and photoelectrocolorimeter have been in good correlation. The described technique is recommended as a method of choice.

UI MeSH Term Description Entries
D007770 L-Lactate Dehydrogenase A tetrameric enzyme that, along with the coenzyme NAD+, catalyzes the interconversion of LACTATE and PYRUVATE. In vertebrates, genes for three different subunits (LDH-A, LDH-B and LDH-C) exist. Lactate Dehydrogenase,Dehydrogenase, L-Lactate,Dehydrogenase, Lactate,L Lactate Dehydrogenase
D004796 Clinical Enzyme Tests Analyses for a specific enzyme activity, or of the level of a specific enzyme that is used to assess health and disease risk, for early detection of disease or disease prediction, diagnosis, and change in disease status. Enzyme Tests, Clinical,Clinical Enzyme Test,Enzyme Test, Clinical,Test, Clinical Enzyme,Tests, Clinical Enzyme
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man

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