Biomaterial Database

[Determination of the proteolytic and antiproteolytic activity of bile]. 1989

V P Miroshnichenko, and P S Arshinov

Basing on the known methods for measuring the proteolytic and antiproteolytic activities with the use of N-benzoyl-L-arginine-paranitroanilide, a synthetic substrate, the authors suggest measuring the bile proteolytic and antiproteolytic activities with the use of the same reagents. The examined parameters of the bile are calculated according to the formulae that rule out preliminary plotting of calibration curves. The inhibitory activity is expressed in kontrical antitrypsin activity units, the proteolytic one in the amount of paranitro-aniline per unit of time. Data on the normal proteolytic and antiproteolytic activities of the bile are presented.

UI	MeSH Term	Description	Entries
D010447	Peptide Hydrolases	Hydrolases that specifically cleave the peptide bonds found in PROTEINS and PEPTIDES. Examples of sub-subclasses for this group include EXOPEPTIDASES and ENDOPEPTIDASES.	Peptidase,Peptidases,Peptide Hydrolase,Protease,Proteases,Proteinase,Proteinases,Proteolytic Enzyme,Proteolytic Enzymes,Esteroproteases,Enzyme, Proteolytic,Hydrolase, Peptide
D011480	Protease Inhibitors	Compounds which inhibit or antagonize biosynthesis or actions of proteases (ENDOPEPTIDASES).	Antiprotease,Endopeptidase Inhibitor,Endopeptidase Inhibitors,Peptidase Inhibitor,Peptidase Inhibitors,Peptide Hydrolase Inhibitor,Peptide Hydrolase Inhibitors,Peptide Peptidohydrolase Inhibitor,Peptide Peptidohydrolase Inhibitors,Protease Antagonist,Protease Antagonists,Antiproteases,Protease Inhibitor,Antagonist, Protease,Antagonists, Protease,Hydrolase Inhibitor, Peptide,Hydrolase Inhibitors, Peptide,Inhibitor, Endopeptidase,Inhibitor, Peptidase,Inhibitor, Peptide Hydrolase,Inhibitor, Peptide Peptidohydrolase,Inhibitor, Protease,Inhibitors, Endopeptidase,Inhibitors, Peptidase,Inhibitors, Peptide Hydrolase,Inhibitors, Peptide Peptidohydrolase,Inhibitors, Protease,Peptidohydrolase Inhibitor, Peptide,Peptidohydrolase Inhibitors, Peptide
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D001120	Arginine	An essential amino acid that is physiologically active in the L-form.	Arginine Hydrochloride,Arginine, L-Isomer,DL-Arginine Acetate, Monohydrate,L-Arginine,Arginine, L Isomer,DL Arginine Acetate, Monohydrate,Hydrochloride, Arginine,L Arginine,L-Isomer Arginine,Monohydrate DL-Arginine Acetate
D001586	Benzoylarginine Nitroanilide	A chromogenic substrate that permits direct measurement of peptide hydrolase activity, e.g., papain and trypsin, by colorimetry. The substrate liberates p-nitroaniline as a chromogenic product.	BAPNA,N-alpha-Benzoyl-DL-arginine-4-nitroanilide,Benzoylarginine Nitroanilide Monohydrochloride,Benzoylarginine Nitroanilide, (R)-Isomer,Benzoylarginine Nitroanilide, (S)-Isomer,Benzoylarginine Nitroanilide, Monosodium Salt, Monohydrochloride,N-Benzoylarginine-4-nitroanilide,N-Benzoylarginyl-4-nitroanilide,Monohydrochloride, Benzoylarginine Nitroanilide,N Benzoylarginine 4 nitroanilide,N Benzoylarginyl 4 nitroanilide,N alpha Benzoyl DL arginine 4 nitroanilide,Nitroanilide, Benzoylarginine
D001646	Bile	An emulsifying agent produced in the LIVER and secreted into the DUODENUM. Its composition includes BILE ACIDS AND SALTS; CHOLESTEROL; and ELECTROLYTES. It aids DIGESTION of fats in the duodenum.	Biliary Sludge,Sludge, Biliary