Induction of functional tissue-engineered skeletal muscle constructs by defined electrical stimulation. 2014

Akira Ito, and Yasunori Yamamoto, and Masanori Sato, and Kazushi Ikeda, and Masahiro Yamamoto, and Hideaki Fujita, and Eiji Nagamori, and Yoshinori Kawabe, and Masamichi Kamihira
1] Department of Chemical Engineering, Faculty of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan [2].

Electrical impulses are necessary for proper in vivo skeletal muscle development. To fabricate functional skeletal muscle tissues in vitro, recapitulation of the in vivo niche, including physical stimuli, is crucial. Here, we report a technique to engineer skeletal muscle tissues in vitro by electrical pulse stimulation (EPS). Electrically excitable tissue-engineered skeletal muscle constructs were stimulated with continuous electrical pulses of 0.3 V/mm amplitude, 4 ms width, and 1 Hz frequency, resulting in a 4.5-fold increase in force at day 14. In myogenic differentiation culture, the percentage of peak twitch force (%Pt) was determined as the load on the tissue constructs during the artificial exercise induced by continuous EPS. We optimized the stimulation protocol, wherein the tissues were first subjected to 24.5%Pt, which was increased to 50-60%Pt as the tissues developed. This technique may be a useful approach to fabricate tissue-engineered functional skeletal muscle constructs.

UI MeSH Term Description Entries
D009119 Muscle Contraction A process leading to shortening and/or development of tension in muscle tissue. Muscle contraction occurs by a sliding filament mechanism whereby actin filaments slide inward among the myosin filaments. Inotropism,Muscular Contraction,Contraction, Muscle,Contraction, Muscular,Contractions, Muscle,Contractions, Muscular,Inotropisms,Muscle Contractions,Muscular Contractions
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D004558 Electric Stimulation Use of electric potential or currents to elicit biological responses. Stimulation, Electric,Electrical Stimulation,Electric Stimulations,Electrical Stimulations,Stimulation, Electrical,Stimulations, Electric,Stimulations, Electrical
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013997 Time Factors Elements of limited time intervals, contributing to particular results or situations. Time Series,Factor, Time,Time Factor
D046509 Tissue Culture Techniques A technique for maintaining or growing TISSUE in vitro, usually by DIFFUSION, perifusion, or PERFUSION. The tissue is cultured directly after removal from the host without being dispersed for cell culture. Culture Technique, Tissue,Culture Techniques, Tissue,Tissue Culture Technique
D051379 Mice The common name for the genus Mus. Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus
D018482 Muscle, Skeletal A subtype of striated muscle, attached by TENDONS to the SKELETON. Skeletal muscles are innervated and their movement can be consciously controlled. They are also called voluntary muscles. Anterior Tibial Muscle,Gastrocnemius Muscle,Muscle, Voluntary,Plantaris Muscle,Skeletal Muscle,Soleus Muscle,Muscle, Anterior Tibial,Muscle, Gastrocnemius,Muscle, Plantaris,Muscle, Soleus,Muscles, Skeletal,Muscles, Voluntary,Skeletal Muscles,Tibial Muscle, Anterior,Voluntary Muscle,Voluntary Muscles
D023822 Tissue Engineering Generating tissue in vitro for clinical applications, such as replacing wounded tissues or impaired organs. The use of TISSUE SCAFFOLDING enables the generation of complex multi-layered tissues and tissue structures. Engineering, Tissue
D032446 Myoblasts Embryonic (precursor) cells of the myogenic lineage that develop from the MESODERM. They undergo proliferation, migrate to their various sites, and then differentiate into the appropriate form of myocytes (MYOCYTES, SKELETAL; MYOCYTES, CARDIAC; MYOCYTES, SMOOTH MUSCLE). Muscle Cells, Embryonic,Muscle Cells, Precursor,Embryonic Muscle Cell,Embryonic Muscle Cells,Muscle Cell, Embryonic,Muscle Cell, Precursor,Myoblast,Precursor Muscle Cell,Precursor Muscle Cells

Related Publications

Akira Ito, and Yasunori Yamamoto, and Masanori Sato, and Kazushi Ikeda, and Masahiro Yamamoto, and Hideaki Fujita, and Eiji Nagamori, and Yoshinori Kawabe, and Masamichi Kamihira
March 2020, Biomaterials,
Akira Ito, and Yasunori Yamamoto, and Masanori Sato, and Kazushi Ikeda, and Masahiro Yamamoto, and Hideaki Fujita, and Eiji Nagamori, and Yoshinori Kawabe, and Masamichi Kamihira
May 2012, Tissue engineering. Part C, Methods,
Akira Ito, and Yasunori Yamamoto, and Masanori Sato, and Kazushi Ikeda, and Masahiro Yamamoto, and Hideaki Fujita, and Eiji Nagamori, and Yoshinori Kawabe, and Masamichi Kamihira
March 2016, Regenerative therapy,
Akira Ito, and Yasunori Yamamoto, and Masanori Sato, and Kazushi Ikeda, and Masahiro Yamamoto, and Hideaki Fujita, and Eiji Nagamori, and Yoshinori Kawabe, and Masamichi Kamihira
January 2006, In vitro cellular & developmental biology. Animal,
Akira Ito, and Yasunori Yamamoto, and Masanori Sato, and Kazushi Ikeda, and Masahiro Yamamoto, and Hideaki Fujita, and Eiji Nagamori, and Yoshinori Kawabe, and Masamichi Kamihira
April 2019, Biomaterials,
Akira Ito, and Yasunori Yamamoto, and Masanori Sato, and Kazushi Ikeda, and Masahiro Yamamoto, and Hideaki Fujita, and Eiji Nagamori, and Yoshinori Kawabe, and Masamichi Kamihira
November 2002, American journal of physiology. Cell physiology,
Akira Ito, and Yasunori Yamamoto, and Masanori Sato, and Kazushi Ikeda, and Masahiro Yamamoto, and Hideaki Fujita, and Eiji Nagamori, and Yoshinori Kawabe, and Masamichi Kamihira
November 2006, Tissue engineering,
Akira Ito, and Yasunori Yamamoto, and Masanori Sato, and Kazushi Ikeda, and Masahiro Yamamoto, and Hideaki Fujita, and Eiji Nagamori, and Yoshinori Kawabe, and Masamichi Kamihira
January 2014, Regenerative medicine,
Akira Ito, and Yasunori Yamamoto, and Masanori Sato, and Kazushi Ikeda, and Masahiro Yamamoto, and Hideaki Fujita, and Eiji Nagamori, and Yoshinori Kawabe, and Masamichi Kamihira
June 2013, Journal of tissue engineering and regenerative medicine,
Akira Ito, and Yasunori Yamamoto, and Masanori Sato, and Kazushi Ikeda, and Masahiro Yamamoto, and Hideaki Fujita, and Eiji Nagamori, and Yoshinori Kawabe, and Masamichi Kamihira
January 2010, Journal of applied biomaterials & biomechanics : JABB,
Copied contents to your clipboard!