Biomaterial Database

Characterization of sperm agglutinating monoclonal antibody and purification of the human sperm antigen. 1986

Y C Yan, and L F Wang, and S S Koide

Population Council, New York, New York.

A monoclonal antibody capable of inducing tail-to-tail agglutination of human sperm was purified by ammonium sulfate fractionation and chromatography on DEAE-cellulose. A 75- to 100-fold purification was achieved. The antibody was of the IgG class and composed of gamma 1 heavy chain and lambda light chains. The purified preparation was effective in inducing sperm agglutination at a minimum concentration of 0.55 microgram/mL and was active after storage at 4 degrees C for over 3 months in the presence of sodium azide. The sperm antigen interacting with this YWK-1 antibody was purified by immunoaffinity chromatography on Sepharose 4B conjugated with YWK-1 antibodies. Two interacting proteins were isolated, with estimated Mr of 84 and 20 kD. The 84 kD protein had an isoelectric point in the range of pH 5.7 to 6.1, and probably lacks carbohydrate moieties. The 20 and 84 kD components may share common antigenic determinants.

UI	MeSH Term	Description	Entries
D008297	Male		Males
D002460	Cell Line	Established cell cultures that have the potential to propagate indefinitely.	Cell Lines,Line, Cell,Lines, Cell
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000911	Antibodies, Monoclonal	Antibodies produced by a single clone of cells.	Monoclonal Antibodies,Monoclonal Antibody,Antibody, Monoclonal
D000918	Antibody Specificity	The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.	Antibody Specificities,Specificities, Antibody,Specificity, Antibody
D000939	Epitopes	Sites on an antigen that interact with specific antibodies.	Antigenic Determinant,Antigenic Determinants,Antigenic Specificity,Epitope,Determinant, Antigenic,Determinants, Antigenic,Specificity, Antigenic
D000954	Antigens, Surface	Antigens on surfaces of cells, including infectious or foreign cells or viruses. They are usually protein-containing groups on cell membranes or walls and may be isolated.	Cell Surface Antigens,Surface Antigens,Surface Markers, Immunological,Cell Surface Antigen,Immunologic Surface Markers,Markers, Immunological Surface,Surface Antigen,Surface Markers, Immunologic,Antigen, Cell Surface,Antigen, Surface,Antigens, Cell Surface,Immunological Surface Markers,Markers, Immunologic Surface,Surface Antigen, Cell,Surface Antigens, Cell
D013073	Sperm Agglutination	Agglutination of spermatozoa by antibodies or autoantibodies.	Agglutination, Sperm,Agglutinations, Sperm,Sperm Agglutinations
D013094	Spermatozoa	Mature male germ cells derived from SPERMATIDS. As spermatids move toward the lumen of the SEMINIFEROUS TUBULES, they undergo extensive structural changes including the loss of cytoplasm, condensation of CHROMATIN into the SPERM HEAD, formation of the ACROSOME cap, the SPERM MIDPIECE and the SPERM TAIL that provides motility.	Sperm,Spermatozoon,X-Bearing Sperm,X-Chromosome-Bearing Sperm,Y-Bearing Sperm,Y-Chromosome-Bearing Sperm,Sperm, X-Bearing,Sperm, X-Chromosome-Bearing,Sperm, Y-Bearing,Sperm, Y-Chromosome-Bearing,Sperms, X-Bearing,Sperms, X-Chromosome-Bearing,Sperms, Y-Bearing,Sperms, Y-Chromosome-Bearing,X Bearing Sperm,X Chromosome Bearing Sperm,X-Bearing Sperms,X-Chromosome-Bearing Sperms,Y Bearing Sperm,Y Chromosome Bearing Sperm,Y-Bearing Sperms,Y-Chromosome-Bearing Sperms