Biomaterial Database

Chemical deglycosylation on a micro-scale of membrane glycoproteins with retention of phosphoryl-protein linkages. 1989

E Horvath, and A M Edwards, and J C Bell, and P E Braun

McGill University, Department of Biochemistry, Montreal, Quebec, Canada.

We provide here an easy and convenient procedure for the chemical deglycosylation of membrane glycoproteins that can be done rapidly and inexpensively on large numbers of membrane samples on a microscale without prior isolation of the glycoprotein in question. We have used the procedure successfully on three different glycoproteins and additionally have shown that covalent linkages of the polypeptide to phosphoryl residues are preserved.

UI	MeSH Term	Description	Entries
D008562	Membrane Glycoproteins	Glycoproteins found on the membrane or surface of cells.	Cell Surface Glycoproteins,Surface Glycoproteins,Cell Surface Glycoprotein,Membrane Glycoprotein,Surface Glycoprotein,Glycoprotein, Cell Surface,Glycoprotein, Membrane,Glycoprotein, Surface,Glycoproteins, Cell Surface,Glycoproteins, Membrane,Glycoproteins, Surface,Surface Glycoprotein, Cell,Surface Glycoproteins, Cell
D008698	Mesylates	Organic salts or esters of methanesulfonic acid.	Mesilate,Methanesulfonates,Mesilates,Mesylate,Methylenesulfonates
D009185	Myelin Proteins	MYELIN-specific proteins that play a structural or regulatory role in the genesis and maintenance of the lamellar MYELIN SHEATH structure.	Myelin Protein,Protein, Myelin,Proteins, Myelin
D010750	Phosphoproteins		Phosphoprotein
D006031	Glycosylation	The synthetic chemistry reaction or enzymatic reaction of adding carbohydrate or glycosyl groups. GLYCOSYLTRANSFERASES carry out the enzymatic glycosylation reactions. The spontaneous, non-enzymatic attachment of reducing sugars to free amino groups in proteins, lipids, or nucleic acids is called GLYCATION (see MAILLARD REACTION).	Protein Glycosylation,Glycosylation, Protein
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D051379	Mice	The common name for the genus Mus.	Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus
D018992	Myelin-Associated Glycoprotein	A myelin protein found in the periaxonal membrane of both the central and peripheral nervous systems myelin sheaths. It binds to cells surface receptors found on AXONS and may regulate cellular interactions between MYELIN and AXONS.	Large Myelin-Associated Glycoprotein Isoform,Sialic Acid Binding Ig-Like Lectin 4A,Sialic Acid Binding Ig-like Lectin 4,Siglec-4,Small Myelin-Associated Glycoprotein Isoform,Large Myelin Associated Glycoprotein Isoform,Myelin Associated Glycoprotein,Sialic Acid Binding Ig Like Lectin 4A,Sialic Acid Binding Ig like Lectin 4,Siglec 4,Small Myelin Associated Glycoprotein Isoform