Biomaterial Database

Binding of 3H-spiperone to human peripheral lymphocytes: absence of stereospecific high-affinity binding. 1989

A Feenstra, and M A Coggiano, and R J Wyatt

Neuropsychiatry Branch, National Institute of Mental Health Neurosciences Center, Washington, DC 20032.

The binding of 3H-spiperone to human peripheral lymphocytes (PBL) was characterized. The (+)-butaclamol displaceable binding of 3H-spiperone was not saturable, and both (+) and (-)-butaclamol were equally potent in displacing 3H-spiperone binding. We did not find evidence for the existence of a high-affinity specific binding site of 3H-spiperone on human PBL.

UI	MeSH Term	Description	Entries
D008214	Lymphocytes	White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS.	Lymphoid Cells,Cell, Lymphoid,Cells, Lymphoid,Lymphocyte,Lymphoid Cell
D011869	Radioligand Assay	Quantitative determination of receptor (binding) proteins in body fluids or tissue using radioactively labeled binding reagents (e.g., antibodies, intracellular receptors, plasma binders).	Protein-Binding Radioassay,Radioreceptor Assay,Assay, Radioligand,Assay, Radioreceptor,Assays, Radioligand,Assays, Radioreceptor,Protein Binding Radioassay,Protein-Binding Radioassays,Radioassay, Protein-Binding,Radioassays, Protein-Binding,Radioligand Assays,Radioreceptor Assays
D011954	Receptors, Dopamine	Cell-surface proteins that bind dopamine with high affinity and trigger intracellular changes influencing the behavior of cells.	Dopamine Receptors,Dopamine Receptor,Receptor, Dopamine
D002069	Butaclamol	A benzocycloheptapyridoisoquinolinol that has been used as an antipsychotic, especially in schizophrenia.	AY-23,028,Butaclamol Hydrochloride,AY 23,028,AY23,028,Hydrochloride, Butaclamol
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D001667	Binding, Competitive	The interaction of two or more substrates or ligands with the same binding site. The displacement of one by the other is used in quantitative and selective affinity measurements.	Competitive Binding
D013134	Spiperone	A spiro butyrophenone analog similar to HALOPERIDOL and other related compounds. It has been recommended in the treatment of SCHIZOPHRENIA.	Spiroperidol,Spiroperone