[Quantitation of hemagglutinins by the planimetry of precipitated erythrocyte patterns. 4. Application to Lewis blood group determination from saliva and urine specimens and their stains]. 1989

H Kimura, and H Suzuki, and S Matsuzawa

Planimetric hemagglutination (PMHA) (Jpn. J. Legal Med., 42, 292-301, 1988) with glutaraldehyde-treated indicator erythrocytes was used to read results of the forensic medical elution test (ET) for the Lewis blood grouping of body fluids. Two variations of PMHA-ET technique were used to determine the Lewis blood group from saliva, urine and their dried stains. In the first technique, saliva, urine samples or saliva extracts from dried stains were coated to the well of a microtiter-plate and then were sensitized with anti-Lea or anti-Leb monoclonal antibodies; these were then followed by antibody elution by heating at 55 degrees C for 30 minutes. This method detected the Lea antigen in 1:5, 120-10, 240 diluted Le (a+) saliva, Leb antigen in 1:10, 240-20, 480 diluted Le (b+) saliva, and detect Lea or Leb antigen of extracts from saliva stains as small as 0.5 X 0.5 cm in size. All 65 saliva samples of Lewis blood groups, 31 Le (a+b-), 29 Le (a-b+) and 5 Le (a-b-), were easily determined and 17 out of 18 urine samples without concentration were also typed. In the second technique, anti-Le antibodies were added to the chopped dried urine stains with no antigen extraction. This technique detected Lea or Leb antigen in the stains 1 X 2 cm in size. The PMHA-ET seems to be more useful for quantitative analysis of the Lewis substances in body fluids than any other conventional methods; this is because of its simplicity, sensitivity and good reproducibility.

UI MeSH Term Description Entries
D007983 Lewis Blood Group Antigens Carbohydrate antigens structurally related to the ABH BLOOD-GROUP SYSTEM. They may occur as a modification of saccharide chains on glycolipids or glycoproteins on cell surfaces or in plasma, or as free oligosaccharides in secretions. Lewis antigens are not synthesized in blood cells. Instead Lewis glycolipids present in plasma are absorbed onto the surface of ERYTHROCYTES; LYMPHOCYTES; and PLATELETS. The phenotypes Le(a) and Le(b) are the result of the actions of two genes the Le gene (fucosyltransferase FUT3) and the Se gene (fucosyltransferase FUT2) on the precursor carbohydrate, glycolipid or glycoprotein. Other FUCOSYLTRANSFERASES can also synthesize the Lewis antigens. Blood Group Lewis Related Antigens,Lewis Antigen Related Tumor-Associated Antigens,Lewis Antigens,Lewis Blood Group Related Antigens,Lewis Blood Group Related Tumor-Associated Antigens,Lewis Blood-Group System,Lewis Related Antigens,Lewis System,Sialyl Lewis Antigens,Le Antigens,Le(a) Blood Group System,Antigens, Lewis,Antigens, Lewis Related,Antigens, Sialyl Lewis,Blood-Group System, Lewis,Lewis Antigen Related Tumor Associated Antigens,Lewis Blood Group Related Tumor Associated Antigens,Lewis Blood Group System
D005554 Forensic Medicine The application of medical knowledge to questions of law. Legal Medicine,Medicine, Forensic,Medicine, Legal
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D012463 Saliva The clear, viscous fluid secreted by the SALIVARY GLANDS and mucous glands of the mouth. It contains MUCINS, water, organic salts, and ptylin. Salivas
D013194 Staining and Labeling The marking of biological material with a dye or other reagent for the purpose of identifying and quantitating components of tissues, cells or their extracts. Histological Labeling,Staining,Histological Labelings,Labeling and Staining,Labeling, Histological,Labelings, Histological,Stainings
D014556 Urine Liquid by-product of excretion produced in the kidneys, temporarily stored in the bladder until discharge through the URETHRA.

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