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The structural composition of erythrocyte ghosts was analysed in children affected by steroid-responsive (SRNS) and unresponsive nephrotic syndrome (SUNS). No variation of either intrinsic or extrinsic ghost proteins was found by discontinuous SDS-electrophoresis associated with a very sensitive double staining technique. By contrast, the composition of inner-layer phospholipids--phosphatidyl ethanolamine (PE) and phosphatidyl serine (PS)--was altered in SRNS with minor changes also involving phosphatidic acid, phosphatidyl inositol and lysophosphatidyl choline. Signs of peroxidative damage were present in both SRNS and SUNS ghosts and inside the cells; these included high levels of fluorescent amino-iminopropene derivates of PE and PS, increased intraerythrocytic amounts of malonyldialdehyde and decreased levels of reduced glutathione. Taken together these results support the concept that in SRNS and SUNS erythrocytes are target cells for peroxidative damage. In SRNS peroxidation of membrane lipids results in a marked alteration of the phospholipid composition of erythrocyte ghosts.
F Ginevri, and
G M Ghiggeri, and
G Candiano, and
R Oleggini, and
R Bertelli, and
M T Piccardo, and
F Perfumo, and
R Gusmano
May 1999,
Pediatric nephrology (Berlin, Germany),
F Ginevri, and
G M Ghiggeri, and
G Candiano, and
R Oleggini, and
R Bertelli, and
M T Piccardo, and
F Perfumo, and
R Gusmano
January 1983,
The American journal of clinical nutrition,
F Ginevri, and
G M Ghiggeri, and
G Candiano, and
R Oleggini, and
R Bertelli, and
M T Piccardo, and
F Perfumo, and
R Gusmano
March 1997,
General pharmacology,
F Ginevri, and
G M Ghiggeri, and
G Candiano, and
R Oleggini, and
R Bertelli, and
M T Piccardo, and
F Perfumo, and
R Gusmano
December 1952,
A.M.A. American journal of diseases of children,
F Ginevri, and
G M Ghiggeri, and
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M T Piccardo, and
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R Gusmano
December 1988,
Irish journal of medical science,
F Ginevri, and
G M Ghiggeri, and
G Candiano, and
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R Bertelli, and
M T Piccardo, and
F Perfumo, and
R Gusmano
January 1991,
Journal of neurochemistry,
F Ginevri, and
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G Candiano, and
R Oleggini, and
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M T Piccardo, and
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R Gusmano
June 1983,
Kurinikaru sutadi = Clinical study,
F Ginevri, and
G M Ghiggeri, and
G Candiano, and
R Oleggini, and
R Bertelli, and
M T Piccardo, and
F Perfumo, and
R Gusmano
June 1983,
Kurinikaru sutadi = Clinical study,
F Ginevri, and
G M Ghiggeri, and
G Candiano, and
R Oleggini, and
R Bertelli, and
M T Piccardo, and
F Perfumo, and
R Gusmano
November 1992,
Cancer research,
F Ginevri, and
G M Ghiggeri, and
G Candiano, and
R Oleggini, and
R Bertelli, and
M T Piccardo, and
F Perfumo, and
R Gusmano
January 1989,
La Ricerca in clinica e in laboratorio,
