-transparent.png){kind=logo}
The covalently linked antigenic complex, bovine serum albumin-alpha-linolenic acid, was used to immunize Balb/c mice against the hapten. Hybridization between splenocytes and the myeloma cell line, P 3 X63 Ag 8,651, resulted in stable clones synthesizing monoclonal antibodies (Mab) that were subsequently purified and characterized. Four Mab (A, B, C, D) were retained and their specificities studied by ELISA. Antibody D only recognized 18-carbon fatty acids having a cis,cis,-cis-1,4,7 unsaturated system in the omega-3 position: it was specific for alpha-linolenic acid. B recognized all fatty acids containing the structure cis,cis,cis-1,4,7-octatriene. A and C recognized polyunsaturated fatty acids with a degree of unsaturation superior to two double bonds.
F Buffière, and
J Cook-Moreau, and
N Gualde, and
M Rigaud
October 1987,
Journal of steroid biochemistry,
F Buffière, and
J Cook-Moreau, and
N Gualde, and
M Rigaud
February 1989,
Lancet (London, England),
F Buffière, and
J Cook-Moreau, and
N Gualde, and
M Rigaud
February 2001,
Molecular biotechnology,
F Buffière, and
J Cook-Moreau, and
N Gualde, and
M Rigaud
July 1988,
Journal of immunological methods,
F Buffière, and
J Cook-Moreau, and
N Gualde, and
M Rigaud
January 2002,
Voprosy meditsinskoi khimii,
F Buffière, and
J Cook-Moreau, and
N Gualde, and
M Rigaud
January 1980,
Lancet (London, England),
F Buffière, and
J Cook-Moreau, and
N Gualde, and
M Rigaud
January 1991,
Biomedica biochimica acta,
F Buffière, and
J Cook-Moreau, and
N Gualde, and
M Rigaud
November 1981,
Biochimica et biophysica acta,
F Buffière, and
J Cook-Moreau, and
N Gualde, and
M Rigaud
January 1998,
Methods in molecular biology (Clifton, N.J.),
F Buffière, and
J Cook-Moreau, and
N Gualde, and
M Rigaud
January 1991,
Bioprocess technology,
