Biomaterial Database

Secretion of thromboxane B2 and prostaglandin E2 by guinea-pig lung cells isolated by centrifugal elutriation and by macrophages obtained by bronchoalveolar lavage. 1989

J P Pelé, and C Robidoux, and P Sirois

Department of Pharmacology, Faculty of Medicine, University of Sherbrooke, Canada.

Guinea-pig lung cells were partially purified by an elutriation technique after enzymatic digestion of the lung. Eight fractions were obtained and the cell content of each fraction was analysed by staining and by electron microscopy. After stimulation with selected agonists, the secretion of eicosanoids has been measured in the supernatants of each elutriation fraction and in the supernatants of alveolar macrophages obtained by bronchoalveolar lavage. The cell response was characterised by a marked secretion of thromboxane B2 (TXB2) and prostaglandin E2 (PGE2) from the alveolar macrophages and a more discrete secretion of TXB2 from the cells of the elutriated fractions. The secretion of TXB2 varied in the cell fractions as a function of the cellular composition and the agonist used. Platelet activating factor (PAF) and ethoxy PAF, phorbol myristate acetate (PMA) and ionophore A-23187 stimulated most cell types although PMA produced a more important stimulation of the fractions containing pneumocytes and eosinophils, and ionophore A-23187 appeared to stimulate Foa-Kurloff cells. The chemotactic peptide fMLP stimulated markedly the fractions containing the inflammatory cells (mast cells, macrophages, neutrophils) and endothelial cells. Pretreatment of the cells with cytochalasin B potentiated the release of TXB2 and PGE2 by macrophages following stimulation with PAF, leukotriene D4 (LTD4) and formyl-methionyl-leucyl-phenylalanine (fMLP) but not by mixed lung cells or by elutriation fractions. In conclusion, the recovery of a large number of partially purified lung cell types was made possible by mild protease digestion and elutriation.(ABSTRACT TRUNCATED AT 250 WORDS)

UI	MeSH Term	Description	Entries
D008168	Lung	Either of the pair of organs occupying the cavity of the thorax that effect the aeration of the blood.	Lungs
D008854	Microscopy, Electron	Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.	Electron Microscopy
D001992	Bronchoalveolar Lavage Fluid	Washing liquid obtained from irrigation of the lung, including the BRONCHI and the PULMONARY ALVEOLI. It is generally used to assess biochemical, inflammatory, or infection status of the lung.	Alveolar Lavage Fluid,Bronchial Lavage Fluid,Lung Lavage Fluid,Bronchial Alveolar Lavage Fluid,Lavage Fluid, Bronchial,Lavage Fluid, Lung,Pulmonary Lavage Fluid,Alveolar Lavage Fluids,Bronchial Lavage Fluids,Bronchoalveolar Lavage Fluids,Lavage Fluid, Alveolar,Lavage Fluid, Bronchoalveolar,Lavage Fluid, Pulmonary,Lavage Fluids, Alveolar,Lavage Fluids, Bronchial,Lavage Fluids, Bronchoalveolar,Lavage Fluids, Lung,Lavage Fluids, Pulmonary,Lung Lavage Fluids,Pulmonary Lavage Fluids
D002498	Centrifugation	Process of using a rotating machine to generate centrifugal force to separate substances of different densities, remove moisture, or simulate gravitational effects. It employs a large motor-driven apparatus with a long arm, at the end of which human and animal subjects, biological specimens, or equipment can be revolved and rotated at various speeds to study gravitational effects. (From Websters, 10th ed; McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
D003584	Cytological Techniques	Methods used to study CELLS.	Cytologic Technics,Cytological Technic,Cytological Technics,Cytological Technique,Technic, Cytological,Technics, Cytological,Technique, Cytological,Techniques, Cytological,Cytologic Technic,Technic, Cytologic,Technics, Cytologic
D006168	Guinea Pigs	A common name used for the genus Cavia. The most common species is Cavia porcellus which is the domesticated guinea pig used for pets and biomedical research.	Cavia,Cavia porcellus,Guinea Pig,Pig, Guinea,Pigs, Guinea
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D013194	Staining and Labeling	The marking of biological material with a dye or other reagent for the purpose of identifying and quantitating components of tissues, cells or their extracts.	Histological Labeling,Staining,Histological Labelings,Labeling and Staining,Labeling, Histological,Labelings, Histological,Stainings
D013268	Stimulation, Chemical	The increase in a measurable parameter of a PHYSIOLOGICAL PROCESS, including cellular, microbial, and plant; immunological, cardiovascular, respiratory, reproductive, urinary, digestive, neural, musculoskeletal, ocular, and skin physiological processes; or METABOLIC PROCESS, including enzymatic and other pharmacological processes, by a drug or other chemical.	Chemical Stimulation,Chemical Stimulations,Stimulations, Chemical
D013929	Thromboxane B2	A stable, physiologically active compound formed in vivo from the prostaglandin endoperoxides. It is important in the platelet-release reaction (release of ADP and serotonin).	B2, Thromboxane