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A double immunofluorescence method was developed for the monitoring of proliferation and differentiation of F9 embryonal carcinoma cells. Cytokeratin filament expression was used as a marker for differentiation, and proliferating cell nuclear antigen (PCNA)/cyclin or bromodeoxyuridine labeling were used as markers for proliferation. F9 cells had a high proliferation rate and were cytokeratin-filament-negative. Upon treatment with retinoic acid and dibutyryl cyclic AMP, cytokeratin-filament-positive cells with differentiated phenotype appeared. After 3 days, the extent of proliferation of cytokeratin-filament-positive cells was comparable to, but after 5 days significantly lower than, that of cytokeratin-filament-negative cells in the same culture. In differentiating F9 cells, cytokeratin filament expression is associated with, and even slightly precedes, the dramatic decrease in the rate of proliferation.
P Kurki, and
A Laasonen, and
E M Tan, and
E Lehtonen
October 1987,
Journal of virology,
P Kurki, and
A Laasonen, and
E M Tan, and
E Lehtonen
December 1987,
Experimental cell research,
P Kurki, and
A Laasonen, and
E M Tan, and
E Lehtonen
January 1989,
Biochemical Society symposium,
P Kurki, and
A Laasonen, and
E M Tan, and
E Lehtonen
April 1990,
Developmental biology,
P Kurki, and
A Laasonen, and
E M Tan, and
E Lehtonen
August 1989,
Molecular and cellular biology,
P Kurki, and
A Laasonen, and
E M Tan, and
E Lehtonen
December 1984,
Cell differentiation,
P Kurki, and
A Laasonen, and
E M Tan, and
E Lehtonen
February 1987,
Experimental cell research,
P Kurki, and
A Laasonen, and
E M Tan, and
E Lehtonen
November 1989,
Genes & development,
P Kurki, and
A Laasonen, and
E M Tan, and
E Lehtonen
September 1992,
Journal of cell science,
P Kurki, and
A Laasonen, and
E M Tan, and
E Lehtonen
January 1982,
Immunogenetics,
