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In the present prospective study we have evaluated the sensitivity, specificity and predictive value of the Bengal rose and indirect immunofluorescence (IIF) in 122 patients with a bacteriological diagnosis of brucellosis. The sensitivity of the Bengal rose was 95.79% and its specificity 98.43%. IIF had a sensitivity of 68.80% and a specificity of 100% when the polyvalent anti-IgS conjugate was used. The combined parallel use of Bengal rose and IIF-IgS resulted in a sensitivity of 89.17% and a specificity of 100%. There was a good correlation between Bengal rose and serum agglutination test (r = 0.72); on the contrary, there were not good correlations between the fluorescent conjugates and Bengal rose, agglutination and Coombs test. In conclusion, the combined use of Bengal rose as a screening test and IIF as a confirmation study is a good diagnostic strategy for human brucellosis.
J D Colmenero, and
J M Reguera, and
F P Cabrera, and
S Hernández, and
J Porras, and
P Manchado, and
M T Miranda
October 1984,
Revista clinica espanola,
J D Colmenero, and
J M Reguera, and
F P Cabrera, and
S Hernández, and
J Porras, and
P Manchado, and
M T Miranda
July 1987,
Veterinarni medicina,
J D Colmenero, and
J M Reguera, and
F P Cabrera, and
S Hernández, and
J Porras, and
P Manchado, and
M T Miranda
January 2010,
Journal of immunoassay & immunochemistry,
J D Colmenero, and
J M Reguera, and
F P Cabrera, and
S Hernández, and
J Porras, and
P Manchado, and
M T Miranda
December 1969,
The Veterinary record,
J D Colmenero, and
J M Reguera, and
F P Cabrera, and
S Hernández, and
J Porras, and
P Manchado, and
M T Miranda
January 1988,
Revista clinica espanola,
J D Colmenero, and
J M Reguera, and
F P Cabrera, and
S Hernández, and
J Porras, and
P Manchado, and
M T Miranda
January 1976,
Veterinariia,
J D Colmenero, and
J M Reguera, and
F P Cabrera, and
S Hernández, and
J Porras, and
P Manchado, and
M T Miranda
September 1974,
Tropical and geographical medicine,
J D Colmenero, and
J M Reguera, and
F P Cabrera, and
S Hernández, and
J Porras, and
P Manchado, and
M T Miranda
January 1971,
Przeglad epidemiologiczny,
J D Colmenero, and
J M Reguera, and
F P Cabrera, and
S Hernández, and
J Porras, and
P Manchado, and
M T Miranda
January 1986,
Polskie archiwum weterynaryjne,
J D Colmenero, and
J M Reguera, and
F P Cabrera, and
S Hernández, and
J Porras, and
P Manchado, and
M T Miranda
November 1980,
Australian veterinary journal,
