-transparent.png){kind=logo}
Guanine- 5-carboxylcytosine base pairs mimic mismatches during DNA replication. 2014
Toshihiro Shibutani, and
Shinsuke Ito, and
Mariko Toda, and
Rie Kanao, and
Leonard B Collins, and
Marika Shibata, and
Miho Urabe, and
Haruhiko Koseki, and
Yuji Masuda, and
James A Swenberg, and
Chikahide Masutani, and
Fumio Hanaoka, and
Shigenori Iwai, and
Isao Kuraoka
Graduate School of Engineering Science, Osaka University Graduate School of Engineering Science, 1-3 Machikaneyama, Toyonaka, Osaka 560-8531 Japan.
The genetic information encoded in genomes must be faithfully replicated and transmitted to daughter cells. The recent discovery of consecutive DNA conversions by TET family proteins of 5-methylcytosine into 5-hydroxymethylcytosine, 5-formylcytosine, and 5-carboxylcytosine (5caC) suggests these modified cytosines act as DNA lesions, which could threaten genome integrity. Here, we have shown that although 5caC pairs with guanine during DNA replication in vitro, G·5caC pairs stimulated DNA polymerase exonuclease activity and were recognized by the mismatch repair (MMR) proteins. Knockdown of thymine DNA glycosylase increased 5caC in genome, affected cell proliferation via MMR, indicating MMR is a novel reader for 5caC. These results suggest the epigenetic modification products of 5caC behave as DNA lesions.
Related Publications
Toshihiro Shibutani, and
Shinsuke Ito, and
Mariko Toda, and
Rie Kanao, and
Leonard B Collins, and
Marika Shibata, and
Miho Urabe, and
Haruhiko Koseki, and
Yuji Masuda, and
James A Swenberg, and
Chikahide Masutani, and
Fumio Hanaoka, and
Shigenori Iwai, and
Isao Kuraoka
December 1984,
Carcinogenesis,
Toshihiro Shibutani, and
Shinsuke Ito, and
Mariko Toda, and
Rie Kanao, and
Leonard B Collins, and
Marika Shibata, and
Miho Urabe, and
Haruhiko Koseki, and
Yuji Masuda, and
James A Swenberg, and
Chikahide Masutani, and
Fumio Hanaoka, and
Shigenori Iwai, and
Isao Kuraoka
December 1987,
Cell,
Toshihiro Shibutani, and
Shinsuke Ito, and
Mariko Toda, and
Rie Kanao, and
Leonard B Collins, and
Marika Shibata, and
Miho Urabe, and
Haruhiko Koseki, and
Yuji Masuda, and
James A Swenberg, and
Chikahide Masutani, and
Fumio Hanaoka, and
Shigenori Iwai, and
Isao Kuraoka
February 2015,
Biochemistry,
Toshihiro Shibutani, and
Shinsuke Ito, and
Mariko Toda, and
Rie Kanao, and
Leonard B Collins, and
Marika Shibata, and
Miho Urabe, and
Haruhiko Koseki, and
Yuji Masuda, and
James A Swenberg, and
Chikahide Masutani, and
Fumio Hanaoka, and
Shigenori Iwai, and
Isao Kuraoka
March 2005,
Journal of the American Chemical Society,
Toshihiro Shibutani, and
Shinsuke Ito, and
Mariko Toda, and
Rie Kanao, and
Leonard B Collins, and
Marika Shibata, and
Miho Urabe, and
Haruhiko Koseki, and
Yuji Masuda, and
James A Swenberg, and
Chikahide Masutani, and
Fumio Hanaoka, and
Shigenori Iwai, and
Isao Kuraoka
April 2024,
IEEE transactions on nanobioscience,
Toshihiro Shibutani, and
Shinsuke Ito, and
Mariko Toda, and
Rie Kanao, and
Leonard B Collins, and
Marika Shibata, and
Miho Urabe, and
Haruhiko Koseki, and
Yuji Masuda, and
James A Swenberg, and
Chikahide Masutani, and
Fumio Hanaoka, and
Shigenori Iwai, and
Isao Kuraoka
December 2019,
Journal of chemical theory and computation,
Toshihiro Shibutani, and
Shinsuke Ito, and
Mariko Toda, and
Rie Kanao, and
Leonard B Collins, and
Marika Shibata, and
Miho Urabe, and
Haruhiko Koseki, and
Yuji Masuda, and
James A Swenberg, and
Chikahide Masutani, and
Fumio Hanaoka, and
Shigenori Iwai, and
Isao Kuraoka
October 2010,
The journal of physical chemistry. A,
Toshihiro Shibutani, and
Shinsuke Ito, and
Mariko Toda, and
Rie Kanao, and
Leonard B Collins, and
Marika Shibata, and
Miho Urabe, and
Haruhiko Koseki, and
Yuji Masuda, and
James A Swenberg, and
Chikahide Masutani, and
Fumio Hanaoka, and
Shigenori Iwai, and
Isao Kuraoka
February 2017,
Nature structural & molecular biology,
Toshihiro Shibutani, and
Shinsuke Ito, and
Mariko Toda, and
Rie Kanao, and
Leonard B Collins, and
Marika Shibata, and
Miho Urabe, and
Haruhiko Koseki, and
Yuji Masuda, and
James A Swenberg, and
Chikahide Masutani, and
Fumio Hanaoka, and
Shigenori Iwai, and
Isao Kuraoka
April 2015,
Biochemistry,
Toshihiro Shibutani, and
Shinsuke Ito, and
Mariko Toda, and
Rie Kanao, and
Leonard B Collins, and
Marika Shibata, and
Miho Urabe, and
Haruhiko Koseki, and
Yuji Masuda, and
James A Swenberg, and
Chikahide Masutani, and
Fumio Hanaoka, and
Shigenori Iwai, and
Isao Kuraoka
March 2015,
Cell research,
