Biomaterial Database

Cloning of midecamycin biosynthetic genes from Streptomyces mycarofaciens 1748. 1989

Y G Wang, and C R Hutchinson

Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences.

A genomic library from the midecamycin producing strain Streptomyces mycarofaciens 1748 was constructed in the Escherichia coli-Streptomyces shuttle cosmid vector pNJ1. Several clones were obtained with DNA homologous to polyketide synthase genes by using the Streptomyces coelicolor actI and actIII genes as hybridization probes. Restriction analysis of the plasmids pCN8B12, pCN6C5 and pCN11E11 showed that their molecular weights were 36, 48.5 and 41.6 kilobase, respectively, and that they contained overlapping regions of DNA. The regions homologous to the actI and actIII genes were localized by DNA hybridization. Transformation of the cloned DNA into the S. mycarofaciens blocked mutant 68 and into Streptomyces lividans TK24 resulted in the production of midecamycin-like substances by TLC and HPLC analyses.

UI	MeSH Term	Description	Entries
D007933	Leucomycins	An antibiotic complex produced by Streptomyces kitasatoensis. The complex consists of a mixture of at least eight biologically active components, A1 and A3 to A9. Leucomycins have both antibacterial and antimycoplasmal activities.
D008970	Molecular Weight	The sum of the weight of all the atoms in a molecule.	Molecular Weights,Weight, Molecular,Weights, Molecular
D009097	Multienzyme Complexes	Systems of enzymes which function sequentially by catalyzing consecutive reactions linked by common metabolic intermediates. They may involve simply a transfer of water molecules or hydrogen atoms and may be associated with large supramolecular structures such as MITOCHONDRIA or RIBOSOMES.	Complexes, Multienzyme
D009693	Nucleic Acid Hybridization	Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)	Genomic Hybridization,Acid Hybridization, Nucleic,Acid Hybridizations, Nucleic,Genomic Hybridizations,Hybridization, Genomic,Hybridization, Nucleic Acid,Hybridizations, Genomic,Hybridizations, Nucleic Acid,Nucleic Acid Hybridizations
D002851	Chromatography, High Pressure Liquid	Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.	Chromatography, High Performance Liquid,Chromatography, High Speed Liquid,Chromatography, Liquid, High Pressure,HPLC,High Performance Liquid Chromatography,High-Performance Liquid Chromatography,UPLC,Ultra Performance Liquid Chromatography,Chromatography, High-Performance Liquid,High-Performance Liquid Chromatographies,Liquid Chromatography, High-Performance
D003001	Cloning, Molecular	The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.	Molecular Cloning
D005285	Fermentation	Anaerobic degradation of GLUCOSE or other organic nutrients to gain energy in the form of ATP. End products vary depending on organisms, substrates, and enzymatic pathways. Common fermentation products include ETHANOL and LACTIC ACID.	Fermentations
D005798	Genes, Bacterial	The functional hereditary units of BACTERIA.	Bacterial Gene,Bacterial Genes,Gene, Bacterial
D013302	Streptomyces	A genus of bacteria that form a nonfragmented aerial mycelium. Many species have been identified with some being pathogenic. This genus is responsible for producing a majority of the ANTI-BACTERIAL AGENTS of practical value.
D014169	Transformation, Bacterial	The heritable modification of the properties of a competent bacterium by naked DNA from another source. The uptake of naked DNA is a naturally occuring phenomenon in some bacteria. It is often used as a GENE TRANSFER TECHNIQUE.	Bacterial Transformation

Related Publications

Y G Wang, and C R Hutchinson

Subcloning and expression of midecamycin polyketide synthase genes from Streptomyces mycarofaciens 1748.

January 1991, Chinese journal of biotechnology,

Y G Wang, and C R Hutchinson

Plasmid DNA in midecamycin-producing strain Streptomyces mycarofaciens 1748.

January 1986, Proceedings of the Chinese Academy of Medical Sciences and the Peking Union Medical College = Chung-kuo i hsueh k'o hsueh yuan, Chung-kuo hsieh ho i k'o ta hsueh hsueh pao,

Y G Wang, and C R Hutchinson

Characterization of polyketide ketoreductase gene (MPKR) from midecamycin-producing strain (Streptomyces mycarofaciens 1748).

January 1994, Chinese journal of biotechnology,

Y G Wang, and C R Hutchinson

Cloning of midecamycin(MLS)-resistance genes from Streptomyces mycarofaciens, Streptomyces lividans and Streptomyces coelicolor A3(2).

August 1990, The Journal of antibiotics,

Y G Wang, and C R Hutchinson

Cloning and characterization of genes encoded in dTDP-D-mycaminose biosynthetic pathway from a midecamycin-producing strain, Streptomyces mycarofaciens.

March 2007, Acta biochimica et biophysica Sinica,

Y G Wang, and C R Hutchinson

[Cloning of DNA fragments containing promoter activity from Streptomyces mycarofaciens 1748].

December 1994, Wei sheng wu xue bao = Acta microbiologica Sinica,