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Basic calcium phosphate (BCP) crystals control the traverse of cells from the G0-G1 to S-phase of the cell cycle and initiate proliferation by rendering fibroblasts competent to respond to insulin-like growth factors in plasma. The present study examines whether BCP crystals induce transcription of the protooncogenes c-fos and c-myc and the effect of beta-interferon (IFN-beta) on protooncogene transcription as well as BCP crystal-induced DNA synthesis. Stimulation of density-arrested BALB/c-3T3 cells with either BCP crystal or platelet-derived growth factor (PDGF) results in maximal accumulation of c-fos mRNA at 30 min after stimulation. Induction of c-myc transcription by BCP crystal or PDGF occurs within 1 h and is maximal at around 3 h after stimulation. Simultaneous addition of IFN-beta with either BCP crystals or PDGF had little effect on c-fos induction but delayed both c-myc message accumulation and entry into S phase. The delay in c-myc message induction after IFN-beta treatment cannot account for the observed delay in the onset of DNA synthesis, since IFN-beta can be added at up to 6 h after stimulation with either PDGF or BCP crystals, and a similar delay in the onset of DNA synthesis is still observed.
H S Cheung, and
P G Mitchell, and
W J Pledger
April 1988,
Science (New York, N.Y.),
H S Cheung, and
P G Mitchell, and
W J Pledger
September 1988,
Molecular endocrinology (Baltimore, Md.),
H S Cheung, and
P G Mitchell, and
W J Pledger
May 1994,
The American journal of physiology,
H S Cheung, and
P G Mitchell, and
W J Pledger
January 1990,
Virchows Archiv. B, Cell pathology including molecular pathology,
H S Cheung, and
P G Mitchell, and
W J Pledger
April 1992,
Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme,
H S Cheung, and
P G Mitchell, and
W J Pledger
April 1993,
Toxicology letters,
H S Cheung, and
P G Mitchell, and
W J Pledger
May 1989,
Blood,
H S Cheung, and
P G Mitchell, and
W J Pledger
May 1988,
Genes & development,
H S Cheung, and
P G Mitchell, and
W J Pledger
August 1993,
The American journal of physiology,
H S Cheung, and
P G Mitchell, and
W J Pledger
March 2003,
Archives of toxicology,
