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Unambiguous typing for HLA-DQ TA10 and 2B3 specificities using specific oligonucleotide probes. 1989
M J Kenter, and
J D Anholts, and
G M Schreuder, and
M C van Eggermond, and
G M Ghyselen, and
J J van Rood, and
M J Giphart
Department of Immunohaematology, University Hospital, Leiden, The Netherlands.
Oligonucleotide probes specific for the serologically defined TA10 and 2B3 specificities were selected based on a comparison of the available HLA-DQ beta sequences. Panel and family segregation studies confirm a complete correlation between the reactivities of the selected probes and the TA10/IIB3 antibodies. The Glu residue at position 45 of the HLA-DQ beta chain is specific for the TA10 determinants, and a DQ beta Gly-Val-Tyr sequence is found at position 45-47 for all 2B3-positive DQ beta chains.
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