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The genes that encode the two subunits of Bacillus subtilis phenylalanyl-tRNA synthetase were cloned from alpha lambda library of chromosomal B. subtilis DNA by specific complementation of a thermosensitive Escherichia coli pheS mutation. Both genes (we named them pheS and pheT, analogous to the corresponding genes of E. coli) are carried by a 6.6-kilobase-pair PstI fragment which also complements E. coli pheT mutations. This fragment directs the synthesis of two proteins identical in size to the purified alpha and beta subunits of the phenylalanyl-tRNA synthetase of B. subtilis with Mrs of 42,000 and 97,000, respectively. A recombinant shuttle plasmid carrying the genes caused 10-fold overproduction of functional phenylalanyl-tRNA synthetase in B. subtilis.
A A Brakhage, and
H Putzer, and
K Shazand, and
R J Röschenthaler, and
M Grunberg-Manago
February 1990,
Biochemistry and cell biology = Biochimie et biologie cellulaire,
A A Brakhage, and
H Putzer, and
K Shazand, and
R J Röschenthaler, and
M Grunberg-Manago
January 2010,
Sheng wu gong cheng xue bao = Chinese journal of biotechnology,
A A Brakhage, and
H Putzer, and
K Shazand, and
R J Röschenthaler, and
M Grunberg-Manago
January 1986,
Gene,
A A Brakhage, and
H Putzer, and
K Shazand, and
R J Röschenthaler, and
M Grunberg-Manago
July 1982,
European journal of biochemistry,
A A Brakhage, and
H Putzer, and
K Shazand, and
R J Röschenthaler, and
M Grunberg-Manago
January 1986,
Biochimie,
A A Brakhage, and
H Putzer, and
K Shazand, and
R J Röschenthaler, and
M Grunberg-Manago
May 1988,
Archives of biochemistry and biophysics,
A A Brakhage, and
H Putzer, and
K Shazand, and
R J Röschenthaler, and
M Grunberg-Manago
March 2002,
Protein expression and purification,
A A Brakhage, and
H Putzer, and
K Shazand, and
R J Röschenthaler, and
M Grunberg-Manago
January 1979,
Journal of bacteriology,
A A Brakhage, and
H Putzer, and
K Shazand, and
R J Röschenthaler, and
M Grunberg-Manago
October 1979,
Journal of medicinal chemistry,
A A Brakhage, and
H Putzer, and
K Shazand, and
R J Röschenthaler, and
M Grunberg-Manago
August 1992,
Nucleic acids research,
