-transparent.png){kind=logo}
A dot-enzyme linked immunosorbent assay (dot-ELISA) for the detection of antibodies in bovine brucellosis using soluble antigens extracted from Brucella abortus S-99 is described in which antigen was deposited on a nitrocellulose sheet (0.5 x 0.5 cm) bound to a plastic strip and the unsaturated sites blocked by a solution of spray-dried milk powder. Sera were tested at dilutions of 1:800 and 1:1600, using rabbit anti-bovine-immunoglobulin or protein-A coupled to peroxidase as conjugates and diamino-benzidine as substrate. A positive reaction was clearly indicated by a brown dot on the nitrocellulose sheet. Using antigen-coated, pre-blocked strips, the test could be completed within 45 minutes.
H V Batra, and
P Chand, and
L Ganju, and
R Mukherjee, and
J R Sadana
October 1985,
Journal of immunological methods,
H V Batra, and
P Chand, and
L Ganju, and
R Mukherjee, and
J R Sadana
January 1990,
Veterinary microbiology,
H V Batra, and
P Chand, and
L Ganju, and
R Mukherjee, and
J R Sadana
September 1993,
New Zealand veterinary journal,
H V Batra, and
P Chand, and
L Ganju, and
R Mukherjee, and
J R Sadana
October 1988,
Laboratory animal science,
H V Batra, and
P Chand, and
L Ganju, and
R Mukherjee, and
J R Sadana
January 2002,
Veterinary research,
H V Batra, and
P Chand, and
L Ganju, and
R Mukherjee, and
J R Sadana
October 1987,
Zentralblatt fur Bakteriologie, Mikrobiologie, und Hygiene. Series A, Medical microbiology, infectious diseases, virology, parasitology,
H V Batra, and
P Chand, and
L Ganju, and
R Mukherjee, and
J R Sadana
August 1985,
American journal of veterinary research,
H V Batra, and
P Chand, and
L Ganju, and
R Mukherjee, and
J R Sadana
October 1993,
The Journal of veterinary medical science,
H V Batra, and
P Chand, and
L Ganju, and
R Mukherjee, and
J R Sadana
January 1982,
Veterinarno-meditsinski nauki,
H V Batra, and
P Chand, and
L Ganju, and
R Mukherjee, and
J R Sadana
February 1987,
European journal of clinical microbiology,
