OBJECTIVE To develop a rapid and simple qualitative and quantitative method for determination of acesulfame, saccharin, cyclamate, aspartame, stevioside and neotame in wine by dispersive solid-phase extraction and ultra-fast liquid chromatography coupled with electrospray ionization tandem mass spectrometry (dSPE-UFLC-ESI/MS/MS). METHODS After the sample was extracted and cleaned by dispersive solid-phase extraction using novel magnetic nanomaterials as adsorbents, the separation was performed by UFLC and the cracking was used by the optimized ESI/MS/MS conditions in a negative electrospray ionization mode. Confirmatory detection was carried out by the principle of three qualitative ions and the retention time. RESULTS The interference matrix could be effectively adsorbed by the novel magnetic nanomaterials. The MS/MS fragment ions were reliable and steady under the suitable conditions, the cracking ways to the parent ions and fragment ions were clear. Quantitative ion pairs were m/z 162 --> 82 for acesulfame, m/z 182 --> 42 for saccharin, m/z 178 --> 80 for cyclamate, m/z 293 --> 200 for aspartame, m/z 803 --> 641 for stevioside, m/z 377 --> 200 for neotame. The recoveries were between 83.4% and 104.0% with the relative standard deviations (RSDs) of 1.1% - 4.0%. The limits of quantification (LOQs) were in the range of 0.1 - 5.0 microg/L. CONCLUSIONS The established method was rapid, accurate and sensitive, it is suitable for confirmation for acesulfame, saccharin, cyclamate, aspartame, stevioside and neotame in wine.