[Fluorescence and optical polarization research on the qualitative and quantitative estimation of neutral carbohydrates in the basement membrane of human placental villi]. 1989

C Pfister, and G Scheuner, and N Städtler
Institut für Anatomie des Bereiches Medizin (Charité) der Humboldt-Universität zu Berlin, DDR.

In the trophoblast basement membrane of human placental villi, neutral carbohydrates were investigated by means of fluorescence and polarization optical methods. By a modified fluorescence-PAS-reaction using a Schiff-type reagent substituted with acriflavine, the basement membrane could be stained selectively. The reaction product of known chemical structure is characterized by a typical birefringence that can be measured in the polarized light. The combination of the fluorescence histochemical method with the polarization optical one permits to obtain qualitative and quantitative data for the carbohydrate component and its incorporation and arrangement into the basement membrane at the molecular level.

UI MeSH Term Description Entries
D007202 Indicators and Reagents Substances used for the detection, identification, analysis, etc. of chemical, biological, or pathologic processes or conditions. Indicators are substances that change in physical appearance, e.g., color, at or approaching the endpoint of a chemical titration, e.g., on the passage between acidity and alkalinity. Reagents are substances used for the detection or determination of another substance by chemical or microscopical means, especially analysis. Types of reagents are precipitants, solvents, oxidizers, reducers, fluxes, and colorimetric reagents. (From Grant & Hackh's Chemical Dictionary, 5th ed, p301, p499) Indicator,Reagent,Reagents,Indicators,Reagents and Indicators
D008856 Microscopy, Fluorescence Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye. Fluorescence Microscopy,Immunofluorescence Microscopy,Microscopy, Immunofluorescence,Fluorescence Microscopies,Immunofluorescence Microscopies,Microscopies, Fluorescence,Microscopies, Immunofluorescence
D011048 Polarography An electrochemical technique for measuring the current that flows in solution as a function of an applied voltage. The observed polarographic wave, resulting from the electrochemical response, depends on the way voltage is applied (linear sweep or differential pulse) and the type of electrode used. Usually a mercury drop electrode is used. DC Polarography,Pulse Polarography,Polarography, DC,Polarography, Pulse
D011247 Pregnancy The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH. Gestation,Pregnancies
D001718 Birefringence The property of nonisotropic media, such as crystals, whereby a single incident beam of light traverses the medium as two beams, each plane-polarized, the planes being at right angles to each other. (Cline et al., Dictionary of Visual Science, 4th ed) Birefraction,Double Refraction,Birefractions,Birefringences,Double Refractions,Refraction, Double,Refractions, Double
D002241 Carbohydrates A class of organic compounds composed of carbon, hydrogen, and oxygen in a ratio of Cn(H2O)n. The largest class of organic compounds, including STARCH; GLYCOGEN; CELLULOSE; POLYSACCHARIDES; and simple MONOSACCHARIDES. Carbohydrate
D002824 Chorionic Villi Threadlike vascular projections of the chorion. Chorionic villi may be free or embedded within the DECIDUA forming the site for exchange of substances between fetal and maternal blood (PLACENTA). Placental Villi,Labyrinth of the Placenta,Labyrinthine Placenta,Placental Labyrinth Layer,Chorionic Villus,Labyrinth Layer, Placental,Placental Labyrinth Layers,Placental Villus,Villi, Chorionic,Villi, Placental,Villus, Chorionic,Villus, Placental
D005260 Female Females
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000167 Acriflavine 3,6-Diamino-10-methylacridinium chloride mixt. with 3,6-acridinediamine. Fluorescent dye used as a local antiseptic and also as a biological stain. It intercalates into nucleic acids thereby inhibiting bacterial and viral replication. Xanthacridinum,2,8-Diamino-10-Methylacridinium Chloride Mixture With 2,8-Diaminoacridine

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