BIOMAT Database Logo BIOMAT Database Logo

Effect of synthetic antimicrobial peptides on Naegleria fowleri trophozoites. 2014

Supathra Tiewcharoen, and Watchara Phurttikul, and Jundee Rabablert, and Prasert Auewarakul, and Sittiruk Roytrakul, and Pruksawan Chetanachan, and Thassanant Atithep, and Virach Junnu

We evaluated the effect of tritrpticin, lactoferrin, killer decapeptide and scrambled peptide in vitro against Naegleria fowleri trophozoites compared with amphotericin B. Tritrpticin (100 microg/ml) caused apoptosis of N. fowleri trophozoites (2x10(5) cells/ml), while lactoferrin, killer decapeptide and scrambled peptide did not. On Gormori trichrome staining, tritrpticin affected the elasticity of the surface membrane and reduced the size of the nuclei of N. fowleri trophozoites. The ultrastructure surface membrane and food cup formation of the trophozoites were 100% inhibited. These results are consistent with inhibition of the nfa1, Mp2CL5 of the treated trophozoite, which plays a role in food cup formation. Tritrpticin 100 microg/ml was not toxic against SK-N-MC cells. Our findings suggest tritrpticin has activity against the surface membrane and nfa1 and Mp2CL5 of N. fowleri trophozoites and could be developed as a potential therapeutic agent.

UI MeSH Term Description Entries
D007781 Lactoferrin An iron-binding protein that was originally characterized as a milk protein. It is widely distributed in secretory fluids and is found in the neutrophilic granules of LEUKOCYTES. The N-terminal part of lactoferrin possesses a serine protease which functions to inactivate the TYPE III SECRETION SYSTEM used by bacteria to export virulence proteins for host cell invasion. Lactotransferrin
D008565 Membrane Proteins Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors. Cell Membrane Protein,Cell Membrane Proteins,Cell Surface Protein,Cell Surface Proteins,Integral Membrane Proteins,Membrane-Associated Protein,Surface Protein,Surface Proteins,Integral Membrane Protein,Membrane Protein,Membrane-Associated Proteins,Membrane Associated Protein,Membrane Associated Proteins,Membrane Protein, Cell,Membrane Protein, Integral,Membrane Proteins, Integral,Protein, Cell Membrane,Protein, Cell Surface,Protein, Integral Membrane,Protein, Membrane,Protein, Membrane-Associated,Protein, Surface,Proteins, Cell Membrane,Proteins, Cell Surface,Proteins, Integral Membrane,Proteins, Membrane,Proteins, Membrane-Associated,Proteins, Surface,Surface Protein, Cell
D008855 Microscopy, Electron, Scanning Microscopy in which the object is examined directly by an electron beam scanning the specimen point-by-point. The image is constructed by detecting the products of specimen interactions that are projected above the plane of the sample, such as backscattered electrons. Although SCANNING TRANSMISSION ELECTRON MICROSCOPY also scans the specimen point by point with the electron beam, the image is constructed by detecting the electrons, or their interaction products that are transmitted through the sample plane, so that is a form of TRANSMISSION ELECTRON MICROSCOPY. Scanning Electron Microscopy,Electron Scanning Microscopy,Electron Microscopies, Scanning,Electron Microscopy, Scanning,Electron Scanning Microscopies,Microscopies, Electron Scanning,Microscopies, Scanning Electron,Microscopy, Electron Scanning,Microscopy, Scanning Electron,Scanning Electron Microscopies,Scanning Microscopies, Electron,Scanning Microscopy, Electron
D009447 Neuroblastoma A common neoplasm of early childhood arising from neural crest cells in the sympathetic nervous system, and characterized by diverse clinical behavior, ranging from spontaneous remission to rapid metastatic progression and death. This tumor is the most common intraabdominal malignancy of childhood, but it may also arise from thorax, neck, or rarely occur in the central nervous system. Histologic features include uniform round cells with hyperchromatic nuclei arranged in nests and separated by fibrovascular septa. Neuroblastomas may be associated with the opsoclonus-myoclonus syndrome. (From DeVita et al., Cancer: Principles and Practice of Oncology, 5th ed, pp2099-2101; Curr Opin Oncol 1998 Jan;10(1):43-51) Neuroblastomas
D009842 Oligopeptides Peptides composed of between two and twelve amino acids. Oligopeptide
D010446 Peptide Fragments Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques. Peptide Fragment,Fragment, Peptide,Fragments, Peptide
D002462 Cell Membrane The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells. Plasma Membrane,Cytoplasmic Membrane,Cell Membranes,Cytoplasmic Membranes,Membrane, Cell,Membrane, Cytoplasmic,Membrane, Plasma,Membranes, Cell,Membranes, Cytoplasmic,Membranes, Plasma,Plasma Membranes
D004591 Electrophoresis, Polyacrylamide Gel Electrophoresis in which a polyacrylamide gel is used as the diffusion medium. Polyacrylamide Gel Electrophoresis,SDS-PAGE,Sodium Dodecyl Sulfate-PAGE,Gel Electrophoresis, Polyacrylamide,SDS PAGE,Sodium Dodecyl Sulfate PAGE,Sodium Dodecyl Sulfate-PAGEs
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia

Related Publications

Supathra Tiewcharoen, and Watchara Phurttikul, and Jundee Rabablert, and Prasert Auewarakul, and Sittiruk Roytrakul, and Pruksawan Chetanachan, and Thassanant Atithep, and Virach Junnu
ANTI-AMEBIC ACTIVITY OF DIOSGENIN ON NAEGLERIA FOWLERI TROPHOZOITES.
September 2015, The Southeast Asian journal of tropical medicine and public health,
Supathra Tiewcharoen, and Watchara Phurttikul, and Jundee Rabablert, and Prasert Auewarakul, and Sittiruk Roytrakul, and Pruksawan Chetanachan, and Thassanant Atithep, and Virach Junnu
Effect of clotrimazole on Naegleria fowleri.
June 1975, Journal of clinical pathology,
Supathra Tiewcharoen, and Watchara Phurttikul, and Jundee Rabablert, and Prasert Auewarakul, and Sittiruk Roytrakul, and Pruksawan Chetanachan, and Thassanant Atithep, and Virach Junnu
Neutrophils extracellular traps damage Naegleria fowleri trophozoites opsonized with human IgG.
August 2016, Parasite immunology,
Supathra Tiewcharoen, and Watchara Phurttikul, and Jundee Rabablert, and Prasert Auewarakul, and Sittiruk Roytrakul, and Pruksawan Chetanachan, and Thassanant Atithep, and Virach Junnu
Effect of antimalarial drugs on Naegleria fowleri.
January 1982, The Medical journal of Australia,
Supathra Tiewcharoen, and Watchara Phurttikul, and Jundee Rabablert, and Prasert Auewarakul, and Sittiruk Roytrakul, and Pruksawan Chetanachan, and Thassanant Atithep, and Virach Junnu
Naegleria fowleri.
October 2019, Trends in parasitology,
Supathra Tiewcharoen, and Watchara Phurttikul, and Jundee Rabablert, and Prasert Auewarakul, and Sittiruk Roytrakul, and Pruksawan Chetanachan, and Thassanant Atithep, and Virach Junnu
Naegleria fowleri.
September 2020, The New England journal of medicine,
Supathra Tiewcharoen, and Watchara Phurttikul, and Jundee Rabablert, and Prasert Auewarakul, and Sittiruk Roytrakul, and Pruksawan Chetanachan, and Thassanant Atithep, and Virach Junnu
De Novo Transcriptome Profiling of Naegleria fowleri Trophozoites and Cysts via RNA Sequencing.
January 2023, Pathogens (Basel, Switzerland),
Supathra Tiewcharoen, and Watchara Phurttikul, and Jundee Rabablert, and Prasert Auewarakul, and Sittiruk Roytrakul, and Pruksawan Chetanachan, and Thassanant Atithep, and Virach Junnu
Use of monoclonal antibodies to distinguish pathogenic Naegleria fowleri (cysts, trophozoites, or flagellate forms) from other Naegleria species.
October 1993, Journal of clinical microbiology,
Supathra Tiewcharoen, and Watchara Phurttikul, and Jundee Rabablert, and Prasert Auewarakul, and Sittiruk Roytrakul, and Pruksawan Chetanachan, and Thassanant Atithep, and Virach Junnu
Interaction of secretory immunoglobulin A antibodies with Naegleria fowleri trophozoites and collagen type I.
March 2003, Canadian journal of microbiology,
Supathra Tiewcharoen, and Watchara Phurttikul, and Jundee Rabablert, and Prasert Auewarakul, and Sittiruk Roytrakul, and Pruksawan Chetanachan, and Thassanant Atithep, and Virach Junnu
Contact-independent cell death of human microglial cells due to pathogenic Naegleria fowleri trophozoites.
December 2008, The Korean journal of parasitology,
Copied contents to your clipboard!