Biomaterial Database

Multiresidue analysis of pesticides in vegetables and fruits by supercritical fluid extraction and liquid chromatography-tandem mass spectrometry. 2014

Shizuka Saito-Shida, and Satoru Nemoto, and Rieko Matsuda

National Institute of Health Sciences.

A multiresidue method for analyzing pesticides in vegetables and fruits by supercritical fluid extraction (SFE) and LC-MS/MS was developed. The sample preparation and SFE parameters were optimized for extracting LC-amenable polar and medium-polarity pesticides. High recoveries were achieved for most of the tested pesticides by extracting a 1 : 1 : 1 sample-Celite-anhydrous magnesium sulfate mixture with supercritical carbon dioxide at 16.4 MPa at 40℃ for 30 min with methanol added as a modifier. The recoveries of 117 pesticides fortified with 0.01 mg/kg of each pesticide were 70-120%, and the relative standard deviations were less than 25% for 112 pesticides in tomato and 103 pesticides in cucumber. No significant differences were observed in the residue concentrations determined in real samples by the SFE method and the liquid extraction method (the modified Japanese official method). Higher recoveries of polar pesticides, such as acephate and methamidophos, were achieved by the developed SFE method than by the liquid extraction method.

UI	MeSH Term	Description	Entries
D007306	Insecticides	Pesticides designed to control insects that are harmful to man. The insects may be directly harmful, as those acting as disease vectors, or indirectly harmful, as destroyers of crops, food products, or textile fabrics.	Insecticide
D009946	Organothiophosphorus Compounds	Compounds containing carbon-phosphorus bonds in which the phosphorus component is also bonded to one or more sulfur atoms. Many of these compounds function as CHOLINERGIC AGENTS and as INSECTICIDES.	Compounds, Organothiophosphorus
D010573	Pesticide Residues	Pesticides or their breakdown products remaining in the environment following their normal use or accidental contamination.	Pesticide Residue,Residue, Pesticide,Residues, Pesticide
D002853	Chromatography, Liquid	Chromatographic techniques in which the mobile phase is a liquid.	Liquid Chromatography
D005504	Food Analysis	Measurement and evaluation of the components of substances to be taken as FOOD.	Analysis, Food,Analyses, Food,Food Analyses
D005506	Food Contamination	The presence in food of harmful, unpalatable, or otherwise objectionable foreign substances, e.g. chemicals, microorganisms or diluents, before, during, or after processing or storage.	Food Adulteration,Adulteration, Food,Adulterations, Food,Contamination, Food,Contaminations, Food,Food Adulterations,Food Contaminations
D005638	Fruit	The fleshy or dry ripened ovary of a plant, enclosing the seed or seeds.	Berries,Legume Pod,Plant Aril,Plant Capsule,Aril, Plant,Arils, Plant,Berry,Capsule, Plant,Capsules, Plant,Fruits,Legume Pods,Plant Arils,Plant Capsules,Pod, Legume,Pods, Legume
D014675	Vegetables	A food group comprised of EDIBLE PLANTS or their parts.	Vegetable
D053719	Tandem Mass Spectrometry	A mass spectrometry technique using two (MS/MS) or more mass analyzers. With two in tandem, the precursor ions are mass-selected by a first mass analyzer, and focused into a collision region where they are then fragmented into product ions which are then characterized by a second mass analyzer. A variety of techniques are used to separate the compounds, ionize them, and introduce them to the first mass analyzer. For example, for in GC-MS/MS, GAS CHROMATOGRAPHY-MASS SPECTROMETRY is involved in separating relatively small compounds by GAS CHROMATOGRAPHY prior to injecting them into an ionization chamber for the mass selection.	Mass Spectrometry-Mass Spectrometry,Mass Spectrometry Mass Spectrometry,Mass Spectrometry, Tandem
D063088	Phosphoramides	Amide derivatives of phosphoric acid such as compounds that include the phosphoric triamide (P(