Biomaterial Database

Murine follicular development requires oocyte DICER, but not DROSHA. 2014

Shuiqiao Yuan, and Nicole Ortogero, and Qiuxia Wu, and Huili Zheng, and Wei Yan

Department of Physiology and Cell Biology, University of Nevada School of Medicine, Reno, Nevada.

Both DICER and DROSHA are RNase III enzymes involved in the biogenesis of small noncoding RNAs. DROSHA cleaves the stem-loop portion of the primary miRNAs and produces precursor miRNAs in the nucleus, whereas DICER processes double-stranded RNA precursors into mature miRNAs and endogenous small interference RNAs in the cytoplasm. Selective inactivation of Dicer in growing oocytes of primary follicles leads to female infertility due to oocyte spindle defects. However, it remains unknown if oocyte Dicer expression in the fetal ovary is required for proper follicular development in the postnatal ovary. Moreover, the role of Drosha in folliculogenesis has never been investigated. Here, we report that conditional knockout of Dicer in prophase I oocytes of the fetal ovary led to compromised folliculogenesis, premature ovarian failure, and female infertility in the adult ovary, whereas selective inactivation of Drosha in oocytes of either the fetal or the developing ovary had no effects on normal folliculogenesis and female fertility in adulthood. Our data indicate that oocyte DICER expression in the fetal ovary is required, and oocyte DROSHA is dispensable, for postnatal follicular development and female fertility in adulthood.

UI	MeSH Term	Description	Entries
D008297	Male		Males
D009865	Oocytes	Female germ cells derived from OOGONIA and termed OOCYTES when they enter MEIOSIS. The primary oocytes begin meiosis but are arrested at the diplotene state until OVULATION at PUBERTY to give rise to haploid secondary oocytes or ova (OVUM).	Ovocytes,Oocyte,Ovocyte
D005260	Female		Females
D006080	Ovarian Follicle	An OOCYTE-containing structure in the cortex of the OVARY. The oocyte is enclosed by a layer of GRANULOSA CELLS providing a nourishing microenvironment (FOLLICULAR FLUID). The number and size of follicles vary depending on the age and reproductive state of the female. The growing follicles are divided into five stages: primary, secondary, tertiary, Graafian, and atretic. Follicular growth and steroidogenesis depend on the presence of GONADOTROPINS.	Graafian Follicle,Atretic Follicle,Ovarian Follicles,Atretic Follicles,Follicle, Atretic,Follicle, Graafian,Follicle, Ovarian,Follicles, Atretic,Follicles, Graafian,Follicles, Ovarian,Graafian Follicles
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D015971	Gene Expression Regulation, Enzymologic	Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in enzyme synthesis.	Enzymologic Gene Expression Regulation,Regulation of Gene Expression, Enzymologic,Regulation, Gene Expression, Enzymologic
D016649	Primary Ovarian Insufficiency	Cessation of ovarian function after MENARCHE but before the age of 40, without or with OVARIAN FOLLICLE depletion. It is characterized by the presence of OLIGOMENORRHEA or AMENORRHEA, elevated GONADOTROPINS, and low ESTRADIOL levels. It is a state of female HYPERGONADOTROPIC HYPOGONADISM. Etiologies include genetic defects, autoimmune processes, chemotherapy, radiation, and infections. The most commonly known genetic cause is the expansion of a CGG repeat to 55 to 199 copies in the 5' untranslated region in the X-linked FMR1 gene.	Gonadotropin-Resistant Ovary Syndrome,Ovarian Failure, Premature,Resistant Ovary Syndrome,FMR1-Related Primary Ovarian Insufficiency,Fragile X Premature Ovarian Failure,Fragile X-Associated Primary Ovarian Insufficiency,Hypergonadotropic Ovarian Failure, X-Linked,Premature Ovarian Failure,Premature Ovarian Failure 1,Premature Ovarian Failure, X-Linked,Primary Ovarian Insufficiency, Fragile X-Associated,X-Linked Hypergonadotropic Ovarian Failure,FMR1 Related Primary Ovarian Insufficiency,Fragile X Associated Primary Ovarian Insufficiency,Gonadotropin Resistant Ovary Syndrome,Hypergonadotropic Ovarian Failure, X Linked,Ovarian Insufficiency, Primary,Premature Ovarian Failure, X Linked,Primary Ovarian Insufficiency, Fragile X Associated,X Linked Hypergonadotropic Ovarian Failure
D043244	Ribonuclease III	An endoribonuclease that is specific for double-stranded RNA. It plays a role in POST-TRANSCRIPTIONAL RNA PROCESSING of pre-RIBOSOMAL RNA and a variety of other RNA structures that contain double-stranded regions.	Dicer Enzyme,RNase D,RNase III,Ribonuclease D,Enzyme, Dicer
D051379	Mice	The common name for the genus Mus.	Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus
D053487	DEAD-box RNA Helicases	A large family of RNA helicases that share a common protein motif with the single letter amino acid sequence D-E-A-D (Asp-Glu-Ala-Asp). In addition to RNA helicase activity, members of the DEAD-box family participate in other aspects of RNA metabolism and regulation of RNA function.	DEAD-box RNA Helicase,DEAD Box Helicase p68,p68 DEAD Box Protein,p68 RNA Helicase,DEAD box RNA Helicase,DEAD box RNA Helicases,Helicase, DEAD-box RNA,Helicase, p68 RNA,Helicases, DEAD-box RNA,RNA Helicase, DEAD-box,RNA Helicase, p68,RNA Helicases, DEAD-box