Serotonin activates cell survival and apoptotic death responses in cultured epithelial thyroid cells. 2014

Giuliana Cerulo, and Simona Tafuri, and Valeria De Pasquale, and Silviana Rea, and Simona Romano, and Anna Costagliola, and Rossella Della Morte, and Luigi Avallone, and Luigi Michele Pavone
Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, Naples, Italy; Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, Naples, Italy.

Anatomic and physiological interactions between central serotonergic system and thyroid gland are well established. However, the effects of locally available serotonin on the thyroid functions are poorly known. Here, we first demonstrate the expression of serotonin transporter SERT and 5-HT2A receptor subtype in rat thyroid epithelial cell line FRT both at mRNA and protein levels. In order to investigate the molecular mechanisms of serotonin action, FRT cells were exposed to increasing concentrations of the amine. Low concentrations of serotonin (up to 5 μM) enhanced FRT cell growth, and ERK1/2 and SMAD2/3 phosphorylation. Cell exposure to the selective 5-HT2A receptor agonist DOI recapitulated the effects of 5-HT on ERK1/2 phosphorylation. By contrast, administration of M100907, a specific 5-HT2A receptor inhibitor, prevented 5-HT induced ERK1/2 activation. On the other hand, high doses of serotonin (50 μM up to 1 mM) activated a caspase-3 mediated apoptosis of cells. Overall, our findings demonstrate that low levels of serotonin, interacting with 5-HT2A receptor, are able to activate proliferative signals in the thyroid epithelial cells, while high levels of serotonin cause pro-apoptotic responses, thus suggesting an active role of the amine in the thyroid functions and disorders.

UI MeSH Term Description Entries
D010880 Piperidines A family of hexahydropyridines.
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D002470 Cell Survival The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability. Cell Viability,Cell Viabilities,Survival, Cell,Viabilities, Cell,Viability, Cell
D004847 Epithelial Cells Cells that line the inner and outer surfaces of the body by forming cellular layers (EPITHELIUM) or masses. Epithelial cells lining the SKIN; the MOUTH; the NOSE; and the ANAL CANAL derive from ectoderm; those lining the RESPIRATORY SYSTEM and the DIGESTIVE SYSTEM derive from endoderm; others (CARDIOVASCULAR SYSTEM and LYMPHATIC SYSTEM) derive from mesoderm. Epithelial cells can be classified mainly by cell shape and function into squamous, glandular and transitional epithelial cells. Adenomatous Epithelial Cells,Columnar Glandular Epithelial Cells,Cuboidal Glandular Epithelial Cells,Glandular Epithelial Cells,Squamous Cells,Squamous Epithelial Cells,Transitional Epithelial Cells,Adenomatous Epithelial Cell,Cell, Adenomatous Epithelial,Cell, Epithelial,Cell, Glandular Epithelial,Cell, Squamous,Cell, Squamous Epithelial,Cell, Transitional Epithelial,Cells, Adenomatous Epithelial,Cells, Epithelial,Cells, Glandular Epithelial,Cells, Squamous,Cells, Squamous Epithelial,Cells, Transitional Epithelial,Epithelial Cell,Epithelial Cell, Adenomatous,Epithelial Cell, Glandular,Epithelial Cell, Squamous,Epithelial Cell, Transitional,Epithelial Cells, Adenomatous,Epithelial Cells, Glandular,Epithelial Cells, Squamous,Epithelial Cells, Transitional,Glandular Epithelial Cell,Squamous Cell,Squamous Epithelial Cell,Transitional Epithelial Cell
D005464 Fluorobenzenes Derivatives of BENZENE that contain FLUORINE. Fluorobenzene
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D012701 Serotonin A biochemical messenger and regulator, synthesized from the essential amino acid L-TRYPTOPHAN. In humans it is found primarily in the central nervous system, gastrointestinal tract, and blood platelets. Serotonin mediates several important physiological functions including neurotransmission, gastrointestinal motility, hemostasis, and cardiovascular integrity. Multiple receptor families (RECEPTORS, SEROTONIN) explain the broad physiological actions and distribution of this biochemical mediator. 5-HT,5-Hydroxytryptamine,3-(2-Aminoethyl)-1H-indol-5-ol,Enteramine,Hippophaine,Hydroxytryptamine,5 Hydroxytryptamine
D013961 Thyroid Gland A highly vascularized endocrine gland consisting of two lobes joined by a thin band of tissue with one lobe on each side of the TRACHEA. It secretes THYROID HORMONES from the follicular cells and CALCITONIN from the parafollicular cells thereby regulating METABOLISM and CALCIUM level in blood, respectively. Thyroid,Gland, Thyroid,Glands, Thyroid,Thyroid Glands,Thyroids
D016601 RNA-Binding Proteins Proteins that bind to RNA molecules. Included here are RIBONUCLEOPROTEINS and other proteins whose function is to bind specifically to RNA. Double-Stranded RNA-Binding Protein,Double-Stranded RNA-Binding Proteins,ds RNA-Binding Protein,RNA-Binding Protein,ds RNA-Binding Proteins,Double Stranded RNA Binding Protein,Double Stranded RNA Binding Proteins,Protein, Double-Stranded RNA-Binding,Protein, ds RNA-Binding,RNA Binding Protein,RNA Binding Proteins,RNA-Binding Protein, Double-Stranded,RNA-Binding Protein, ds,RNA-Binding Proteins, Double-Stranded,ds RNA Binding Protein

Related Publications

Giuliana Cerulo, and Simona Tafuri, and Valeria De Pasquale, and Silviana Rea, and Simona Romano, and Anna Costagliola, and Rossella Della Morte, and Luigi Avallone, and Luigi Michele Pavone
May 2014, Biochimica et biophysica acta,
Giuliana Cerulo, and Simona Tafuri, and Valeria De Pasquale, and Silviana Rea, and Simona Romano, and Anna Costagliola, and Rossella Della Morte, and Luigi Avallone, and Luigi Michele Pavone
March 2004, Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie,
Giuliana Cerulo, and Simona Tafuri, and Valeria De Pasquale, and Silviana Rea, and Simona Romano, and Anna Costagliola, and Rossella Della Morte, and Luigi Avallone, and Luigi Michele Pavone
December 1985, The Journal of endocrinology,
Giuliana Cerulo, and Simona Tafuri, and Valeria De Pasquale, and Silviana Rea, and Simona Romano, and Anna Costagliola, and Rossella Della Morte, and Luigi Avallone, and Luigi Michele Pavone
May 2004, Glia,
Giuliana Cerulo, and Simona Tafuri, and Valeria De Pasquale, and Silviana Rea, and Simona Romano, and Anna Costagliola, and Rossella Della Morte, and Luigi Avallone, and Luigi Michele Pavone
November 2008, Placenta,
Giuliana Cerulo, and Simona Tafuri, and Valeria De Pasquale, and Silviana Rea, and Simona Romano, and Anna Costagliola, and Rossella Della Morte, and Luigi Avallone, and Luigi Michele Pavone
February 2017, Biochimica et biophysica acta. General subjects,
Giuliana Cerulo, and Simona Tafuri, and Valeria De Pasquale, and Silviana Rea, and Simona Romano, and Anna Costagliola, and Rossella Della Morte, and Luigi Avallone, and Luigi Michele Pavone
June 2000, The Journal of membrane biology,
Giuliana Cerulo, and Simona Tafuri, and Valeria De Pasquale, and Silviana Rea, and Simona Romano, and Anna Costagliola, and Rossella Della Morte, and Luigi Avallone, and Luigi Michele Pavone
April 2019, Molecular medicine reports,
Giuliana Cerulo, and Simona Tafuri, and Valeria De Pasquale, and Silviana Rea, and Simona Romano, and Anna Costagliola, and Rossella Della Morte, and Luigi Avallone, and Luigi Michele Pavone
August 1998, Cell structure and function,
Giuliana Cerulo, and Simona Tafuri, and Valeria De Pasquale, and Silviana Rea, and Simona Romano, and Anna Costagliola, and Rossella Della Morte, and Luigi Avallone, and Luigi Michele Pavone
July 2004, Infection and immunity,
Copied contents to your clipboard!