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The in vitro spasmolytic activity of glycerol trinitrate was measured on the KCl-contraction of aorta strips from the rabbit. In the presence of sulphobromophthalein, a known inhibitor of glutathione S-transferase, the dose-activity curve for the nitrate was displaced to the right. Much smaller displacements were obtained with the control spasmolytic substances--papaverine and S-nitroso-N-acetylpenicillamine. It was confirmed that sulphobromophthalein inhibits glutathione S-transferase activity in aorta homogenates. Aorta extracts did not detectably catalyze the reaction between glutathione and sulphobromophthalein and the glutathione level was not decreased by treating the intact aorta with sulphobromophthalein. It is concluded that sulphobromophthalein acts as a specific antagonist of the spasmolytic activity of glycerol trinitrate, probably as a result of its inhibition of glutathione S-transferase. It thus seems probable that glutathione and glutathione S-transferase are involved in the pharmacological activation of the organic nitrates.
R A Yeates, and
M Schmid, and
M Leitold
September 2006,
The Journal of pharmacology and experimental therapeutics,
R A Yeates, and
M Schmid, and
M Leitold
February 1993,
Canadian journal of physiology and pharmacology,
R A Yeates, and
M Schmid, and
M Leitold
December 1996,
The Journal of pharmacology and experimental therapeutics,
R A Yeates, and
M Schmid, and
M Leitold
January 2021,
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R A Yeates, and
M Schmid, and
M Leitold
January 1983,
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R A Yeates, and
M Schmid, and
M Leitold
March 1995,
Applied and environmental microbiology,
R A Yeates, and
M Schmid, and
M Leitold
April 1989,
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R A Yeates, and
M Schmid, and
M Leitold
August 1984,
Biochemical pharmacology,
R A Yeates, and
M Schmid, and
M Leitold
April 1979,
Biochemical and biophysical research communications,
R A Yeates, and
M Schmid, and
M Leitold
July 1992,
Arzneimittel-Forschung,
