BIOMAT Database Logo BIOMAT Database Logo

[Selective medium to isolate human Bifidobacterium]. 2014

Dongxue Huo, and Jiachao Zhang, and Na Bai, and Weiqiang Huang, and Heping Zhang

OBJECTIVE To compare five selective media to isolate human Bifidobacterium. METHODS Feces from six healthy human volunteers were diluted and cultivated on five Bifidobacterium selective media. After anaerobic cultivation, bacterial colonies were counted, selected and identified. Meanwhile, bacterial genomic DNA was extracted from the feces samples, and the Denaturing Gradient Gel Electrophoresis (DGGE) and Quantitative Polymerase Chain Reaction (q-PCR) were applied to reveal the diversity of Bifidobacterium. RESULTS The amount of Bifidobacterium grown on BSM and BLM media was similar to the result detected by q-PCR and was significantly higher than that on three other media. Bifidobacterium isolated from BLM medium was similar to the identified result of DGGE profile. CONCLUSIONS BLM medium is the best selective medium for Bifidobacterium isolation from human gastrointestinal tract.

UI MeSH Term Description Entries
D007223 Infant A child between 1 and 23 months of age. Infants
D008297 Male Males
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D010802 Phylogeny The relationships of groups of organisms as reflected by their genetic makeup. Community Phylogenetics,Molecular Phylogenetics,Phylogenetic Analyses,Phylogenetic Analysis,Phylogenetic Clustering,Phylogenetic Comparative Analysis,Phylogenetic Comparative Methods,Phylogenetic Distance,Phylogenetic Generalized Least Squares,Phylogenetic Groups,Phylogenetic Incongruence,Phylogenetic Inference,Phylogenetic Networks,Phylogenetic Reconstruction,Phylogenetic Relatedness,Phylogenetic Relationships,Phylogenetic Signal,Phylogenetic Structure,Phylogenetic Tree,Phylogenetic Trees,Phylogenomics,Analyse, Phylogenetic,Analysis, Phylogenetic,Analysis, Phylogenetic Comparative,Clustering, Phylogenetic,Community Phylogenetic,Comparative Analysis, Phylogenetic,Comparative Method, Phylogenetic,Distance, Phylogenetic,Group, Phylogenetic,Incongruence, Phylogenetic,Inference, Phylogenetic,Method, Phylogenetic Comparative,Molecular Phylogenetic,Network, Phylogenetic,Phylogenetic Analyse,Phylogenetic Clusterings,Phylogenetic Comparative Analyses,Phylogenetic Comparative Method,Phylogenetic Distances,Phylogenetic Group,Phylogenetic Incongruences,Phylogenetic Inferences,Phylogenetic Network,Phylogenetic Reconstructions,Phylogenetic Relatednesses,Phylogenetic Relationship,Phylogenetic Signals,Phylogenetic Structures,Phylogenetic, Community,Phylogenetic, Molecular,Phylogenies,Phylogenomic,Reconstruction, Phylogenetic,Relatedness, Phylogenetic,Relationship, Phylogenetic,Signal, Phylogenetic,Structure, Phylogenetic,Tree, Phylogenetic
D003470 Culture Media Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN. Media, Culture
D005243 Feces Excrement from the INTESTINES, containing unabsorbed solids, waste products, secretions, and BACTERIA of the DIGESTIVE SYSTEM.
D005260 Female Females
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000328 Adult A person having attained full growth or maturity. Adults are of 19 through 44 years of age. For a person between 19 and 24 years of age, YOUNG ADULT is available. Adults
D001644 Bifidobacterium A rod-shaped, gram-positive, non-acid-fast, non-spore-forming, non-motile bacterium that is a genus of the family Bifidobacteriaceae, order Bifidobacteriales, class ACTINOBACTERIA. It inhabits the intestines and feces of humans as well as the human vagina.

Related Publications

Dongxue Huo, and Jiachao Zhang, and Na Bai, and Weiqiang Huang, and Heping Zhang
[Use of milk-hydrolysate medium to isolate Bifidobacterium in intestinal dysbacteriosis].
January 1983, Laboratornoe delo,
Dongxue Huo, and Jiachao Zhang, and Na Bai, and Weiqiang Huang, and Heping Zhang
A new selective medium for Bifidobacterium spp.
November 1999, Applied and environmental microbiology,
Dongxue Huo, and Jiachao Zhang, and Na Bai, and Weiqiang Huang, and Heping Zhang
Failure of a selective medium to isolate Haemophilus somnus strains.
May 1989, Australian veterinary journal,
Dongxue Huo, and Jiachao Zhang, and Na Bai, and Weiqiang Huang, and Heping Zhang
Improved medium for selective isolation and enumeration of Bifidobacterium.
November 1980, Applied and environmental microbiology,
Dongxue Huo, and Jiachao Zhang, and Na Bai, and Weiqiang Huang, and Heping Zhang
Selective medium for isolation and enumeration of Bifidobacterium spp.
July 1988, Applied and environmental microbiology,
Dongxue Huo, and Jiachao Zhang, and Na Bai, and Weiqiang Huang, and Heping Zhang
Use of modified Lactobacillus selective medium and Bifidobacterium iodoacetate medium for differential enumeration of Lactobacillus acidophilus and Bifidobacterium spp. in powdered nutritional products.
January 1999, Journal of food protection,
Dongxue Huo, and Jiachao Zhang, and Na Bai, and Weiqiang Huang, and Heping Zhang
Bifidobacterium-selective isolation and enumeration from chicken caeca by a modified oligosaccharide antibiotic-selective agar medium.
January 2005, Letters in applied microbiology,
Dongxue Huo, and Jiachao Zhang, and Na Bai, and Weiqiang Huang, and Heping Zhang
A novel selective growth medium-PCR assay to isolate and detect Sphingomonas in environmental samples.
July 2010, Journal of microbiological methods,
Dongxue Huo, and Jiachao Zhang, and Na Bai, and Weiqiang Huang, and Heping Zhang
Modified Pagano Levin medium to isolate Candida species.
July 1966, Applied microbiology,
Dongxue Huo, and Jiachao Zhang, and Na Bai, and Weiqiang Huang, and Heping Zhang
An optimized culture medium to isolate Lactobacillus fermentum strains from the human intestinal tract.
August 2021, Food & function,
Copied contents to your clipboard!