Biomaterial Database

Determination of N-acetylation degree in chitosan using Raman spectroscopy. 2015

A Zając, and J Hanuza, and M Wandas, and L Dymińska

Department of Bioorganic Chemistry, Institute of Chemistry and Food Technology, Faculty of Engineering and Economics, Wrocław University of Economics, Komandorska 118/120, 53-345 Wrocław, Poland.

Application of Raman spectroscopy in determination of the acetylation degree (DA) of chitosan has been developed. The spectra of several chitosan samples characterized by different DD (degree of deacetylation) in the range 50-100% have been measured. The integral intensities of the bands assigned to the vibrations of amine group and glucosidic ring were used to calculate the DA from the intensity ratio. The assignment of the bands to the respective normal modes of chitosan was based on the DFT quantum chemical calculations. This method has a number of advantages over other techniques. It is fast and does not require purification of the sample nor require dissolution of the chitosan in any solvent.

UI	MeSH Term	Description	Entries
D008956	Models, Chemical	Theoretical representations that simulate the behavior or activity of chemical processes or phenomena; includes the use of mathematical equations, computers, and other electronic equipment.	Chemical Models,Chemical Model,Model, Chemical
D008958	Models, Molecular	Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.	Molecular Models,Model, Molecular,Molecular Model
D011789	Quantum Theory	The theory that the radiation and absorption of energy take place in definite quantities called quanta (E) which vary in size and are defined by the equation E	Quantum Theories,Theories, Quantum,Theory, Quantum
D000107	Acetylation	Formation of an acetyl derivative. (Stedman, 25th ed)	Acetylations
D013059	Spectrum Analysis, Raman	Analysis of the intensity of Raman scattering of monochromatic light as a function of frequency of the scattered light.	Raman Spectroscopy,Analysis, Raman Spectrum,Raman Optical Activity Spectroscopy,Raman Scattering,Raman Spectrum Analysis,Scattering, Raman,Spectroscopy, Raman
D017550	Spectroscopy, Fourier Transform Infrared	A spectroscopic technique in which a range of wavelengths is presented simultaneously with an interferometer and the spectrum is mathematically derived from the pattern thus obtained.	FTIR,Fourier Transform Infrared Spectroscopy,Spectroscopy, Infrared, Fourier Transform
D048271	Chitosan	Deacetylated CHITIN, a linear polysaccharide of deacetylated beta-1,4-D-glucosamine. It is used in HYDROGEL and to treat WOUNDS.	Poliglusam