Hyperpolization-activated Ca(2+) channels in guard cell plasma membrane are involved in extracellular ATP-promoted stomatal opening in Vicia faba. 2014

Fang Wang, and Juanjuan Jia, and Yufang Wang, and Weixia Wang, and Yuling Chen, and Ting Liu, and Zhonglin Shang
Hebei Key Laboratory of Molecular & Cellular Biology, Key Laboratory of Molecular & Cellular Biology of Ministry of Education, College of Life Sciences, Hebei Normal University, Shijiazhuang 050016, Hebei, PR China.

Extracellular ATP (eATP) plays essential roles in plant growth, development, and stress tolerance. Extracellular ATP-regulated stomatal movement of Arabidopsis thaliana has been reported. Here, ATP was found to promote stomatal opening of Vicia faba in a dose-dependent manner. Three weakly hydrolysable ATP analogs (adenosine 5'-O-(3-thio) triphosphate (ATPγS), 3'-O-(4-benzoyl) benzoyl adenosine 5'-triphosphate (Bz-ATP) and 2-methylthio-adenosine 5'-triphosphate (2meATP)) showed similar effects, indicating that ATP acts as a signal molecule rather than an energy charger. ADP promoted stomatal opening, while AMP and adenosine did not affect stomatal movement. An ATP-promoted stomatal opening was blocked by the NADPH oxidase inhibitor diphenylene iodonium (DPI), the reductant dithiothreitol (DTT) or the Ca(2+) channel blockers GdCl3 and LaCl3. A hyperpolarization-activated Ca(2+) channel was detected in plasma membrane of guard cell protoplast. Extracellular ATP and weakly hydrolyzable ATP analogs activated this Ca(2+) channel significantly. Extracellular ATP-promoted Ca(2+) channel activation was markedly inhibited by DPI or DTT. These results indicated that eATP may promote stomatal opening via reactive oxygen species that regulate guard cell plasma membrane Ca(2+) channels.

UI MeSH Term Description Entries
D010940 Plant Proteins Proteins found in plants (flowers, herbs, shrubs, trees, etc.). The concept does not include proteins found in vegetables for which PLANT PROTEINS, DIETARY is available. Plant Protein,Protein, Plant,Proteins, Plant
D002462 Cell Membrane The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells. Plasma Membrane,Cytoplasmic Membrane,Cell Membranes,Cytoplasmic Membranes,Membrane, Cell,Membrane, Cytoplasmic,Membrane, Plasma,Membranes, Cell,Membranes, Cytoplasmic,Membranes, Plasma,Plasma Membranes
D000255 Adenosine Triphosphate An adenine nucleotide containing three phosphate groups esterified to the sugar moiety. In addition to its crucial roles in metabolism adenosine triphosphate is a neurotransmitter. ATP,Adenosine Triphosphate, Calcium Salt,Adenosine Triphosphate, Chromium Salt,Adenosine Triphosphate, Magnesium Salt,Adenosine Triphosphate, Manganese Salt,Adenylpyrophosphate,CaATP,CrATP,Manganese Adenosine Triphosphate,MgATP,MnATP,ATP-MgCl2,Adenosine Triphosphate, Chromium Ammonium Salt,Adenosine Triphosphate, Magnesium Chloride,Atriphos,Chromium Adenosine Triphosphate,Cr(H2O)4 ATP,Magnesium Adenosine Triphosphate,Striadyne,ATP MgCl2
D015220 Calcium Channels Voltage-dependent cell membrane glycoproteins selectively permeable to calcium ions. They are categorized as L-, T-, N-, P-, Q-, and R-types based on the activation and inactivation kinetics, ion specificity, and sensitivity to drugs and toxins. The L- and T-types are present throughout the cardiovascular and central nervous systems and the N-, P-, Q-, & R-types are located in neuronal tissue. Ion Channels, Calcium,Receptors, Calcium Channel Blocker,Voltage-Dependent Calcium Channel,Calcium Channel,Calcium Channel Antagonist Receptor,Calcium Channel Antagonist Receptors,Calcium Channel Blocker Receptor,Calcium Channel Blocker Receptors,Ion Channel, Calcium,Receptors, Calcium Channel Antagonist,VDCC,Voltage-Dependent Calcium Channels,Calcium Channel, Voltage-Dependent,Calcium Channels, Voltage-Dependent,Calcium Ion Channel,Calcium Ion Channels,Channel, Voltage-Dependent Calcium,Channels, Voltage-Dependent Calcium,Voltage Dependent Calcium Channel,Voltage Dependent Calcium Channels
D017382 Reactive Oxygen Species Molecules or ions formed by the incomplete one-electron reduction of oxygen. These reactive oxygen intermediates include SINGLET OXYGEN; SUPEROXIDES; PEROXIDES; HYDROXYL RADICAL; and HYPOCHLOROUS ACID. They contribute to the microbicidal activity of PHAGOCYTES, regulation of SIGNAL TRANSDUCTION and GENE EXPRESSION, and the oxidative damage to NUCLEIC ACIDS; PROTEINS; and LIPIDS. Active Oxygen Species,Oxygen Radical,Oxygen Radicals,Pro-Oxidant,Reactive Oxygen Intermediates,Active Oxygen,Oxygen Species, Reactive,Pro-Oxidants,Oxygen, Active,Pro Oxidant,Pro Oxidants,Radical, Oxygen
D054046 Plant Stomata Closable openings in the epidermis of plants on the underside of leaves. They allow the exchange of gases between the internal tissues of the plant and the outside atmosphere. Stomata, Plant,Plant Stomatas,Stomatas, Plant
D031307 Vicia faba A plant species of the genus VICIA, family FABACEAE. The edible beans are well known but they cause FAVISM in some individuals with GLUCOSEPHOSPHATE DEHYDROGENASE DEFICIENCY. This plant contains vicine, convicine, Vicia lectins, unknown seed protein, AAP2 transport protein, and Vicia faba DNA-binding protein 1. Bean, Faba,Bean, Horse,Faba Bean,Fava Bean,Horsebean,Bean, Fava,Beans, Faba,Beans, Fava,Beans, Horse,Faba Beans,Fava Beans,Horse Bean,Horse Beans,Horsebeans,Vicia fabas,fabas, Vicia
D064428 Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels A subgroup of cyclic nucleotide-regulated ION CHANNELS of the superfamily of pore-loop cation channels that are opened by hyperpolarization rather than depolarization. The ion conducting pore passes SODIUM, CALCIUM, and POTASSIUM cations with a preference for potassium. Hyperpolarization Cyclic-Nucleotide Gated Ion Channels,HCN Pacemaker Channels,HCN1 Channel,HCN2 Channel,HCN2 Potassium Channel,HCN3 Channel,HCN4 Channel,Hyperpolarization Activated Cyclic Nucleotide-Gated Potassium Channel 2,Hyperpolarization Cyclic-Nucleotide Gated Cation Channel 1,Hyperpolarization Cyclic-Nucleotide Gated Cation Channel 3,Hyperpolarization Cyclic-Nucleotide Gated Cation Channel 4,Hyperpolarization-Activated Cation Channel,I(h) Cation Channels,I(h) Channels,Ih Cation Channels,Potassium-Sodium Hyperpolarization-Activated Cyclic Nucleotide-Gated Channel 2,Cation Channel, Hyperpolarization-Activated,Cation Channels, Ih,Channel, HCN1,Channel, HCN2,Channel, HCN2 Potassium,Channel, HCN3,Channel, HCN4,Channel, Hyperpolarization-Activated Cation,Channels, HCN Pacemaker,Channels, Ih Cation,Hyperpolarization Activated Cation Channel,Hyperpolarization Activated Cyclic Nucleotide Gated Channels,Hyperpolarization Activated Cyclic Nucleotide Gated Potassium Channel 2,Hyperpolarization Cyclic Nucleotide Gated Cation Channel 1,Hyperpolarization Cyclic Nucleotide Gated Cation Channel 3,Hyperpolarization Cyclic Nucleotide Gated Cation Channel 4,Hyperpolarization Cyclic Nucleotide Gated Ion Channels,Pacemaker Channels, HCN,Potassium Channel, HCN2,Potassium Sodium Hyperpolarization Activated Cyclic Nucleotide Gated Channel 2

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