Biomaterial Database

Determination of cell-bound Fc receptors using a cell adherence test (CAT). 1989

H Lemke, and G Huss, and J Bonmann, and H Hansen

Biochemisches Institut, Medizinischen Fakultät, Christian-Albrechts-Universität zu Kiel, F.R.G.

The determination of membrane-bound Fc receptors as rosette forming cells with antibody-coated erythrocytes (EA-RFC) is a tedious and time consuming method. Here, we describe a modification of the assay delineated by Eisenbarth et al. (J. Immunol. Methods (1980) 39, 387) for the detection of Fc receptors on cell surfaces. V-shaped microtiter plates were coated with an antigen (e.g., fluorescein isothiocyanate (FITC) coupled to bovine serum albumin (BSA] and subsequently with monoclonal anti-FITC antibodies of different isotypes. Cells to be tested were added, incubated for a short time or centrifuged immediately. Cells expressing FcR, became bound to the flanks of the V-shaped wells, whereas cells lacking the receptor were pelleted to the tip of the well. This cell adherence test (CAT) is independent of the agglutinating abilities of the antibodies, and since it is very fast and highly reproducible, large numbers of different cells can be screened for FcR expression. Anti-receptor antibodies can also be tested for their binding to FcR.

UI	MeSH Term	Description	Entries
D011961	Receptors, Fc	Molecules found on the surface of some, but not all, B-lymphocytes, T-lymphocytes, and macrophages, which recognize and combine with the Fc (crystallizable) portion of immunoglobulin molecules.	Fc Receptors,Fc Receptor,Receptor, Fc
D002448	Cell Adhesion	Adherence of cells to surfaces or to other cells.	Adhesion, Cell,Adhesions, Cell,Cell Adhesions
D005452	Fluoresceins	A family of spiro(isobenzofuran-1(3H),9'-(9H)xanthen)-3-one derivatives. These are used as dyes, as indicators for various metals, and as fluorescent labels in immunoassays.	Tetraiodofluorescein
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D012397	Rosette Formation	The in vitro formation of clusters consisting of a cell (usually a lymphocyte) surrounded by antigenic cells or antigen-bearing particles (usually erythrocytes, which may or may not be coated with antibody or antibody and complement). The rosette-forming cell may be an antibody-forming cell, a memory cell, a T-cell, a cell bearing surface cytophilic antibodies, or a monocyte possessing Fc receptors. Rosette formation can be used to identify specific populations of these cells.	Immunocytoadherence,Formation, Rosette,Formations, Rosette,Immunocytoadherences,Rosette Formations
D013861	Thiocyanates	Organic derivatives of thiocyanic acid which contain the general formula R-SCN.	Rhodanate,Rhodanates
D016650	Fluorescein-5-isothiocyanate	Fluorescent probe capable of being conjugated to tissue and proteins. It is used as a label in fluorescent antibody staining procedures as well as protein- and amino acid-binding techniques.	FITC,5-Isothiocyanatofluorescein,Fluorescein (5 or 6)-Isothiocyanate,Fluorescein-5-isothiocyanate Hydrochloride,5 Isothiocyanatofluorescein,Fluorescein 5 isothiocyanate,Fluorescein 5 isothiocyanate Hydrochloride,Hydrochloride, Fluorescein-5-isothiocyanate