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BACKGROUND Burkholderia pseudomallei is a facultative intracellular pathogen and the causative agent of melioidosis. A conserved type III secretion system (T3SS3) and type VI secretion system (T6SS1) are critical for intracellular survival and growth. The T3SS3 and T6SS1 genes are coordinately and hierarchically regulated by a TetR-type regulator, BspR. A central transcriptional regulator of the BspR regulatory cascade, BsaN, activates a subset of T3SS3 and T6SS1 loci. RESULTS To elucidate the scope of the BsaN regulon, we used RNAseq analysis to compare the transcriptomes of wild-type B. pseudomallei KHW and a bsaN deletion mutant. The 60 genes positively-regulated by BsaN include those that we had previously identified in addition to a polyketide biosynthesis locus and genes involved in amino acid biosynthesis. BsaN was also found to repress the transcription of 51 genes including flagellar motility loci and those encoding components of the T3SS3 apparatus. Using a promoter-lacZ fusion assay in E. coli, we show that BsaN together with the chaperone BicA directly control the expression of the T3SS3 translocon, effector and associated regulatory genes that are organized into at least five operons (BPSS1516-BPSS1552). Using a mutagenesis approach, a consensus regulatory motif in the promoter regions of BsaN-regulated genes was shown to be essential for transcriptional activation. CONCLUSIONS BsaN/BicA functions as a central regulator of key virulence clusters in B. pseudomallei within a more extensive network of genetic regulation. We propose that BsaN/BicA controls a gene expression program that facilitates the adaption and intracellular survival of the pathogen within eukaryotic hosts.
Yahua Chen, and
Imke Schröder, and
Christopher T French, and
Artur Jaroszewicz, and
Xiao Jie Yee, and
Boon-Eng Teh, and
Isabelle J Toesca, and
Jeff F Miller, and
Yunn-Hwen Gan
January 2014,
TheScientificWorldJournal,
Yahua Chen, and
Imke Schröder, and
Christopher T French, and
Artur Jaroszewicz, and
Xiao Jie Yee, and
Boon-Eng Teh, and
Isabelle J Toesca, and
Jeff F Miller, and
Yunn-Hwen Gan
June 2009,
Journal of proteome research,
Yahua Chen, and
Imke Schröder, and
Christopher T French, and
Artur Jaroszewicz, and
Xiao Jie Yee, and
Boon-Eng Teh, and
Isabelle J Toesca, and
Jeff F Miller, and
Yunn-Hwen Gan
April 1997,
Epidemiology and infection,
Yahua Chen, and
Imke Schröder, and
Christopher T French, and
Artur Jaroszewicz, and
Xiao Jie Yee, and
Boon-Eng Teh, and
Isabelle J Toesca, and
Jeff F Miller, and
Yunn-Hwen Gan
August 2007,
Journal of medical microbiology,
Yahua Chen, and
Imke Schröder, and
Christopher T French, and
Artur Jaroszewicz, and
Xiao Jie Yee, and
Boon-Eng Teh, and
Isabelle J Toesca, and
Jeff F Miller, and
Yunn-Hwen Gan
December 2022,
Virulence,
Yahua Chen, and
Imke Schröder, and
Christopher T French, and
Artur Jaroszewicz, and
Xiao Jie Yee, and
Boon-Eng Teh, and
Isabelle J Toesca, and
Jeff F Miller, and
Yunn-Hwen Gan
March 2004,
The Indian journal of medical research,
Yahua Chen, and
Imke Schröder, and
Christopher T French, and
Artur Jaroszewicz, and
Xiao Jie Yee, and
Boon-Eng Teh, and
Isabelle J Toesca, and
Jeff F Miller, and
Yunn-Hwen Gan
April 2003,
Infection and immunity,
Yahua Chen, and
Imke Schröder, and
Christopher T French, and
Artur Jaroszewicz, and
Xiao Jie Yee, and
Boon-Eng Teh, and
Isabelle J Toesca, and
Jeff F Miller, and
Yunn-Hwen Gan
January 2011,
Frontiers in microbiology,
Yahua Chen, and
Imke Schröder, and
Christopher T French, and
Artur Jaroszewicz, and
Xiao Jie Yee, and
Boon-Eng Teh, and
Isabelle J Toesca, and
Jeff F Miller, and
Yunn-Hwen Gan
August 2002,
Gene,
Yahua Chen, and
Imke Schröder, and
Christopher T French, and
Artur Jaroszewicz, and
Xiao Jie Yee, and
Boon-Eng Teh, and
Isabelle J Toesca, and
Jeff F Miller, and
Yunn-Hwen Gan
March 2005,
Antimicrobial agents and chemotherapy,
