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The rise in serum immunoactive inhibin levels in male rats following hCG stimulation raised the possibility that Leydig cells may produce inhibin. This study therefore evaluated whether Percoll-purified Leydig cells from adult male rats synthesized and secreted inhibin in vitro as measured by Northern blot analysis, radioimmunoassay and in vitro bioassay. Northern blot analysis demonstrated the presence of alpha-inhibin subunit mRNA in the Leydig cell and inhibin bioactivity was detected in Leydig cell culture media. Levels of immunoactive inhibin increased in culture over 20 h and were directly dependent on the number of Leydig cells in culture. rLH (NIADDK-rLH-I-6) and not rFSH (NIADDK-rFSH-I-6) stimulated immunoactive inhibin levels in a dose-dependent manner. This study demonstrates that Leydig cells express mRNA for the alpha-subunit of inhibin and produce inhibin which is biologically and immunologically active prompting a re-evaluation of our concepts of testicular inhibin production.
G P Risbridger, and
J Clements, and
D M Robertson, and
A E Drummond, and
J Muir, and
H G Burger, and
D M de Kretser
February 1992,
Molecular and cellular endocrinology,
G P Risbridger, and
J Clements, and
D M Robertson, and
A E Drummond, and
J Muir, and
H G Burger, and
D M de Kretser
August 1994,
Journal of molecular endocrinology,
G P Risbridger, and
J Clements, and
D M Robertson, and
A E Drummond, and
J Muir, and
H G Burger, and
D M de Kretser
January 1995,
Life sciences,
G P Risbridger, and
J Clements, and
D M Robertson, and
A E Drummond, and
J Muir, and
H G Burger, and
D M de Kretser
December 1999,
Endocrinology,
G P Risbridger, and
J Clements, and
D M Robertson, and
A E Drummond, and
J Muir, and
H G Burger, and
D M de Kretser
August 1998,
The Journal of veterinary medical science,
G P Risbridger, and
J Clements, and
D M Robertson, and
A E Drummond, and
J Muir, and
H G Burger, and
D M de Kretser
March 2002,
The Journal of endocrinology,
G P Risbridger, and
J Clements, and
D M Robertson, and
A E Drummond, and
J Muir, and
H G Burger, and
D M de Kretser
February 2003,
American journal of clinical pathology,
G P Risbridger, and
J Clements, and
D M Robertson, and
A E Drummond, and
J Muir, and
H G Burger, and
D M de Kretser
June 1992,
Molecular and cellular endocrinology,
G P Risbridger, and
J Clements, and
D M Robertson, and
A E Drummond, and
J Muir, and
H G Burger, and
D M de Kretser
July 1990,
Differentiation; research in biological diversity,
G P Risbridger, and
J Clements, and
D M Robertson, and
A E Drummond, and
J Muir, and
H G Burger, and
D M de Kretser
October 1997,
The Journal of endocrinology,
