BIOMAT Database Logo BIOMAT Database Logo

A study of beta protein precursor using antiserum against a synthetic peptide (1-28). 1989

Y Harigaya, and M Shoji, and H Yamaguchi, and S Hirai, and M Takatama
Department of Neurology, Gunma University School of Medicine, Japan.

We studied the presence of the beta protein precursor by immunocytochemistry and Western blot analysis using antiserum against a synthetic beta peptide (1-28). The brains of 4 patients with dementia of the Alzheimer type (DAT) were examined immunocytochemically. Frozen and formic acid pretreated paraffin sections were best for beta protein immunostaining. Both the senile plaque amyloid and cerebrovascular amyloid were immunostained with 28K1. In addition to primitive and typical plaques, amorphous granular or fibrous deposits of amyloid were observed in high numbers in various areas of the DAT brains. However, the beta protein precursor, which was suggested to be present in neuronal cells by in situ hybridization, was not stained. Neurofibrillary tangles were not stained, either. Sera and cerebrospinal fluid (CSF) from 6 patients with DAT were examined using Western blot analysis. A 52-kD band was detected with 28K1 in DAT patients as well as in normal controls. Further investigation revealed the 52-kD band to be the heavy chain of IgG. The staining pattern using the beta protein immunostain was quite different from the IgG immunostain. In this study there was no detection of the beta protein precursor, with the use of immunocytochemistry and Western blot analysis. Thus, further investigations are necessary using the antiserum against other residues of the beta protein precursor or using specimens without IgG.

UI MeSH Term Description Entries
D007150 Immunohistochemistry Histochemical localization of immunoreactive substances using labeled antibodies as reagents. Immunocytochemistry,Immunogold Techniques,Immunogold-Silver Techniques,Immunohistocytochemistry,Immunolabeling Techniques,Immunogold Technics,Immunogold-Silver Technics,Immunolabeling Technics,Immunogold Silver Technics,Immunogold Silver Techniques,Immunogold Technic,Immunogold Technique,Immunogold-Silver Technic,Immunogold-Silver Technique,Immunolabeling Technic,Immunolabeling Technique,Technic, Immunogold,Technic, Immunogold-Silver,Technic, Immunolabeling,Technics, Immunogold,Technics, Immunogold-Silver,Technics, Immunolabeling,Technique, Immunogold,Technique, Immunogold-Silver,Technique, Immunolabeling,Techniques, Immunogold,Techniques, Immunogold-Silver,Techniques, Immunolabeling
D011498 Protein Precursors Precursors, Protein
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000544 Alzheimer Disease A degenerative disease of the BRAIN characterized by the insidious onset of DEMENTIA. Impairment of MEMORY, judgment, attention span, and problem solving skills are followed by severe APRAXIAS and a global loss of cognitive abilities. The condition primarily occurs after age 60, and is marked pathologically by severe cortical atrophy and the triad of SENILE PLAQUES; NEUROFIBRILLARY TANGLES; and NEUROPIL THREADS. (From Adams et al., Principles of Neurology, 6th ed, pp1049-57) Acute Confusional Senile Dementia,Alzheimer's Diseases,Dementia, Alzheimer Type,Dementia, Senile,Presenile Alzheimer Dementia,Senile Dementia, Alzheimer Type,Alzheimer Dementia,Alzheimer Disease, Early Onset,Alzheimer Disease, Late Onset,Alzheimer Sclerosis,Alzheimer Syndrome,Alzheimer Type Senile Dementia,Alzheimer's Disease,Alzheimer's Disease, Focal Onset,Alzheimer-Type Dementia (ATD),Dementia, Presenile,Dementia, Primary Senile Degenerative,Early Onset Alzheimer Disease,Familial Alzheimer Disease (FAD),Focal Onset Alzheimer's Disease,Late Onset Alzheimer Disease,Primary Senile Degenerative Dementia,Senile Dementia, Acute Confusional,Alzheimer Dementias,Alzheimer Disease, Familial (FAD),Alzheimer Diseases,Alzheimer Type Dementia,Alzheimer Type Dementia (ATD),Alzheimers Diseases,Dementia, Alzheimer,Dementia, Alzheimer-Type (ATD),Familial Alzheimer Diseases (FAD),Presenile Dementia,Sclerosis, Alzheimer,Senile Dementia
D000682 Amyloid A fibrous protein complex that consists of proteins folded into a specific cross beta-pleated sheet structure. This fibrillar structure has been found as an alternative folding pattern for a variety of functional proteins. Deposits of amyloid in the form of AMYLOID PLAQUES are associated with a variety of degenerative diseases. The amyloid structure has also been found in a number of functional proteins that are unrelated to disease. Amyloid Fibril,Amyloid Fibrils,Amyloid Substance,Fibril, Amyloid,Fibrils, Amyloid,Substance, Amyloid
D000906 Antibodies Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
D012189 Retrospective Studies Studies used to test etiologic hypotheses in which inferences about an exposure to putative causal factors are derived from data relating to characteristics of persons under study or to events or experiences in their past. The essential feature is that some of the persons under study have the disease or outcome of interest and their characteristics are compared with those of unaffected persons. Retrospective Study,Studies, Retrospective,Study, Retrospective
D015153 Blotting, Western Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes. Immunoblotting, Western,Western Blotting,Western Immunoblotting,Blot, Western,Immunoblot, Western,Western Blot,Western Immunoblot,Blots, Western,Blottings, Western,Immunoblots, Western,Immunoblottings, Western,Western Blots,Western Blottings,Western Immunoblots,Western Immunoblottings
D016564 Amyloid beta-Protein Precursor A single-pass type I membrane protein. It is cleaved by AMYLOID PRECURSOR PROTEIN SECRETASES to produce peptides of varying amino acid lengths. A 39-42 amino acid peptide, AMYLOID BETA-PEPTIDES is a principal component of the extracellular amyloid in SENILE PLAQUES. Amyloid A4 Protein Precursor,Amyloid Protein Precursor,beta-Amyloid Protein Precursor,Amyloid beta Precursor Protein,Protease Nexin 2,Protease Nexin II,Amyloid beta Protein Precursor,Nexin 2, Protease,Nexin II, Protease,beta Amyloid Protein Precursor,beta-Protein Precursor, Amyloid

Related Publications

Y Harigaya, and M Shoji, and H Yamaguchi, and S Hirai, and M Takatama
An antiserum against amyloid beta-protein precursor detects a unique peptide in Alzheimer brain.
May 1989, Neuroscience letters,
Y Harigaya, and M Shoji, and H Yamaguchi, and S Hirai, and M Takatama
Immunohistochemical study of cerebral amyloid angiopathy: use of an antiserum to a synthetic 28-amino-acid peptide fragment of the Alzheimer's disease amyloid precursor.
February 1988, Human pathology,
Y Harigaya, and M Shoji, and H Yamaguchi, and S Hirai, and M Takatama
A human chorionic gonadotropin-specific antiserum against synthetic peptide analogs to the carboxyl-terminal peptide of its beta-subunit.
February 1979, Endocrinology,
Y Harigaya, and M Shoji, and H Yamaguchi, and S Hirai, and M Takatama
Cross-linking of a synthetic partial-length (1-28) peptide of the Alzheimer beta/A4 amyloid protein by transglutaminase.
July 1993, FEBS letters,
Y Harigaya, and M Shoji, and H Yamaguchi, and S Hirai, and M Takatama
Interleukin-1 beta dissociates beta-amyloid precursor protein and beta-amyloid peptide secretion.
November 1994, FEBS letters,
Y Harigaya, and M Shoji, and H Yamaguchi, and S Hirai, and M Takatama
Immunoreactive adrenocorticotropin in rat brain: a neuroanatomical study using antiserum generated against synthetic ACTH.
August 1980, The American journal of anatomy,
Y Harigaya, and M Shoji, and H Yamaguchi, and S Hirai, and M Takatama
Amyloid-like properties of a synthetic peptide corresponding to the carboxy terminus of beta-amyloid protein precursor.
January 1992, Archives of biochemistry and biophysics,
Y Harigaya, and M Shoji, and H Yamaguchi, and S Hirai, and M Takatama
Secreted forms of beta-amyloid precursor protein protect hippocampal neurons against amyloid beta-peptide-induced oxidative injury.
July 1994, Experimental neurology,
Y Harigaya, and M Shoji, and H Yamaguchi, and S Hirai, and M Takatama
A comparative immunocytochemical study using an antiserum against a synthetic analogue of the corpora cardiaca peptide Pea-CAH-I (MI, neurohormone D) of Periplaneta americana.
June 1996, Cell and tissue research,
Y Harigaya, and M Shoji, and H Yamaguchi, and S Hirai, and M Takatama
Antigenic specificity of a rabbit antiserum raised against the 15-28 segment of thymosin alpha 1.
July 1986, Molecular immunology,
Copied contents to your clipboard!