Age- and sex-related differences of organic anion-transporting polypeptide gene expression in livers of rats. 2014

Wei-Yu Hou, and Shang-Fu Xu, and Qiong-Ni Zhu, and Yuan-Fu Lu, and Xing-Guo Cheng, and Jie Liu
Key Lab for Pharmacology of Ministry of Education, Zunyi Medical College, Zunyi 563003, China.

Organic anion-transporting polypeptides (Oatps) play important roles in transporting endogenous substances and xenobiotics into the liver and are implicated in drug-drug interactions. Many factors could influence their expression and result in alterations in drug disposition, efficacy and toxicity. This study was aimed to examine the development-, aging-, and sex-dependent Oatps expression in livers of rats. The livers from SD rats during development (-2, 1, 7, 14, 21, 28, 35, and 60 d) and aging (60, 180, 540 and/or 800 d) were collected and total RNAs were extracted, purified, and subjected to real-time PCR analysis. Total proteins were extracted for western-blot analysis. Results showed that Oatp1a1, Oatp1a4, Oatp1a5 and Oatp1b2 were all hardly detectable in fetal rat livers, low at birth, rapidly increased after weaning (21 d), and reached the peak at 60 d. The Oatps remained stable during the age between 60-180 d, and decreased at elderly (540 and/or 800 d). After birth, Oatp1a1, Oatp1a4, and Oatp1b2 were all highly expressed in liver, in contrast, Oatp1a5 expression was low. Oatp expressions are male-predominant in rat livers. In the livers of aged rats, the Oatp expression decreased and shared a consistent ontogeny pattern at the mRNA and protein level. In conclusion, this study showed that in rat liver, Oatp1a1, Oatp1a4, Oatp1a5 and Oatp1b2 gene expressions are influenced by age and gender, which could provide a basis of individual variation in drug transport, metabolism and toxicity in children, elderly and women.

UI MeSH Term Description Entries
D008099 Liver A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances. Livers
D005333 Fetus The unborn young of a viviparous mammal, in the postembryonic period, after the major structures have been outlined. In humans, the unborn young from the end of the eighth week after CONCEPTION until BIRTH, as distinguished from the earlier EMBRYO, MAMMALIAN. Fetal Structures,Fetal Tissue,Fetuses,Mummified Fetus,Retained Fetus,Fetal Structure,Fetal Tissues,Fetus, Mummified,Fetus, Retained,Structure, Fetal,Structures, Fetal,Tissue, Fetal,Tissues, Fetal
D000074542 Solute Carrier Organic Anion Transporter Family Member 1B3 A sodium-independent organic anion transporter that functions in the uptake of various drugs and endogenous compounds including ESTRADIOL; TAUROCHOLATE; LEUKOTRIENE C4; and METHOTREXATE. It also functions in clearing BILE ACIDS and organic anions from the liver. Mutations in the SLCO1B3 gene are associated with Rotor Type HYPERBILIRUBINEMIA. Liver-Specific Organic Anion Transporter-2,OATP1B3 Protein,OATP8 Protein,SLC21A8 Protein,SLCO1B3 Protein,Solute Carrier Family 21 Member 8 Protein,Liver Specific Organic Anion Transporter 2
D000367 Age Factors Age as a constituent element or influence contributing to the production of a result. It may be applicable to the cause or the effect of a circumstance. It is used with human or animal concepts but should be differentiated from AGING, a physiological process, and TIME FACTORS which refers only to the passage of time. Age Reporting,Age Factor,Factor, Age,Factors, Age
D000375 Aging The gradual irreversible changes in structure and function of an organism that occur as a result of the passage of time. Senescence,Aging, Biological,Biological Aging
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D012333 RNA, Messenger RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm. Messenger RNA,Messenger RNA, Polyadenylated,Poly(A) Tail,Poly(A)+ RNA,Poly(A)+ mRNA,RNA, Messenger, Polyadenylated,RNA, Polyadenylated,mRNA,mRNA, Non-Polyadenylated,mRNA, Polyadenylated,Non-Polyadenylated mRNA,Poly(A) RNA,Polyadenylated mRNA,Non Polyadenylated mRNA,Polyadenylated Messenger RNA,Polyadenylated RNA,RNA, Polyadenylated Messenger,mRNA, Non Polyadenylated
D012727 Sex Characteristics Those characteristics that distinguish one SEX from the other. The primary sex characteristics are the OVARIES and TESTES and their related hormones. Secondary sex characteristics are those which are masculine or feminine but not directly related to reproduction. Gender Characteristics,Gender Differences,Gender Dimorphism,Sex Differences,Sex Dimorphism,Sexual Dichromatism,Sexual Dimorphism,Characteristic, Gender,Characteristic, Sex,Dichromatism, Sexual,Dichromatisms, Sexual,Difference, Sex,Dimorphism, Gender,Dimorphism, Sex,Dimorphism, Sexual,Gender Characteristic,Gender Difference,Gender Dimorphisms,Sex Characteristic,Sex Difference,Sex Dimorphisms,Sexual Dichromatisms,Sexual Dimorphisms
D017207 Rats, Sprague-Dawley A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company. Holtzman Rat,Rats, Holtzman,Sprague-Dawley Rat,Rats, Sprague Dawley,Holtzman Rats,Rat, Holtzman,Rat, Sprague-Dawley,Sprague Dawley Rat,Sprague Dawley Rats,Sprague-Dawley Rats
D051381 Rats The common name for the genus Rattus. Rattus,Rats, Laboratory,Rats, Norway,Rattus norvegicus,Laboratory Rat,Laboratory Rats,Norway Rat,Norway Rats,Rat,Rat, Laboratory,Rat, Norway,norvegicus, Rattus

Related Publications

Wei-Yu Hou, and Shang-Fu Xu, and Qiong-Ni Zhu, and Yuan-Fu Lu, and Xing-Guo Cheng, and Jie Liu
September 2018, Fluids and barriers of the CNS,
Wei-Yu Hou, and Shang-Fu Xu, and Qiong-Ni Zhu, and Yuan-Fu Lu, and Xing-Guo Cheng, and Jie Liu
October 1997, The Journal of biological chemistry,
Wei-Yu Hou, and Shang-Fu Xu, and Qiong-Ni Zhu, and Yuan-Fu Lu, and Xing-Guo Cheng, and Jie Liu
September 2011, American journal of perinatology,
Wei-Yu Hou, and Shang-Fu Xu, and Qiong-Ni Zhu, and Yuan-Fu Lu, and Xing-Guo Cheng, and Jie Liu
July 2009, The Journal of steroid biochemistry and molecular biology,
Wei-Yu Hou, and Shang-Fu Xu, and Qiong-Ni Zhu, and Yuan-Fu Lu, and Xing-Guo Cheng, and Jie Liu
January 2019, Journal of Nippon Medical School = Nippon Ika Daigaku zasshi,
Wei-Yu Hou, and Shang-Fu Xu, and Qiong-Ni Zhu, and Yuan-Fu Lu, and Xing-Guo Cheng, and Jie Liu
January 2018, Frontiers in pharmacology,
Wei-Yu Hou, and Shang-Fu Xu, and Qiong-Ni Zhu, and Yuan-Fu Lu, and Xing-Guo Cheng, and Jie Liu
May 2002, The Journal of pharmacology and experimental therapeutics,
Wei-Yu Hou, and Shang-Fu Xu, and Qiong-Ni Zhu, and Yuan-Fu Lu, and Xing-Guo Cheng, and Jie Liu
January 2003, Placenta,
Wei-Yu Hou, and Shang-Fu Xu, and Qiong-Ni Zhu, and Yuan-Fu Lu, and Xing-Guo Cheng, and Jie Liu
January 2001, Biochemical and biophysical research communications,
Wei-Yu Hou, and Shang-Fu Xu, and Qiong-Ni Zhu, and Yuan-Fu Lu, and Xing-Guo Cheng, and Jie Liu
March 2009, Pharmacogenomics,
Copied contents to your clipboard!