Biomaterial Database

Characterization of monoclonal antibodies to human interferon-gamma and their application to enzyme-linked immunosorbent assay (ELISA). 1989

M Hirai, and Y Terano, and Y Miura

Suntory Institute for Biomedical Research, Osaka.

The authors obtained two mouse monoclonal antibodies, G-208 and G-166, to recombinant human interferon-gamma (rH-IFN-gamma). Immunologically, they were classified as IgG1-K subclass. G-208 neutralized the antiviral activity of natural and recombinant human IFN-gamma, but did not bind to heat-denatured rH-IFN-gamma. G-166 was able to bind to rH-IFN-gamma as well as to heat-denatured rH-IFN-gamma, but it did not bind to natural human IFN-gamma (nH-IFN-gamma). A sandwich enzyme immunoassay specific to H-IFN-gamma molecule was developed using polyclonal rabbit anti-nH-IFN-gamma antibody and G-208. This assay monitors only biologically active H-IFN-gamma molecule. Thus, this method may be used for the direct determination of H-IFN-gamma instead of determination of antiviral activity of H-IFN-gamma.

UI	MeSH Term	Description	Entries
D007120	Immunochemistry	Field of chemistry that pertains to immunological phenomena and the study of chemical reactions related to antigen stimulation of tissues. It includes physicochemical interactions between antigens and antibodies.
D007371	Interferon-gamma	The major interferon produced by mitogenically or antigenically stimulated LYMPHOCYTES. It is structurally different from TYPE I INTERFERON and its major activity is immunoregulation. It has been implicated in the expression of CLASS II HISTOCOMPATIBILITY ANTIGENS in cells that do not normally produce them, leading to AUTOIMMUNE DISEASES.	Interferon Type II,Interferon, Immune,gamma-Interferon,Interferon, gamma,Type II Interferon,Immune Interferon,Interferon, Type II
D004797	Enzyme-Linked Immunosorbent Assay	An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.	ELISA,Assay, Enzyme-Linked Immunosorbent,Assays, Enzyme-Linked Immunosorbent,Enzyme Linked Immunosorbent Assay,Enzyme-Linked Immunosorbent Assays,Immunosorbent Assay, Enzyme-Linked,Immunosorbent Assays, Enzyme-Linked
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000911	Antibodies, Monoclonal	Antibodies produced by a single clone of cells.	Monoclonal Antibodies,Monoclonal Antibody,Antibody, Monoclonal
D066298	In Vitro Techniques	Methods to study reactions or processes taking place in an artificial environment outside the living organism.	In Vitro Test,In Vitro Testing,In Vitro Tests,In Vitro as Topic,In Vitro,In Vitro Technique,In Vitro Testings,Technique, In Vitro,Techniques, In Vitro,Test, In Vitro,Testing, In Vitro,Testings, In Vitro,Tests, In Vitro,Vitro Testing, In