Anoctamin 1 is apically expressed on thyroid follicular cells and contributes to ATP- and calcium-activated iodide efflux. 2014

Carmela Iosco, and Cristina Cosentino, and Laura Sirna, and Roberta Romano, and Silvia Cursano, and Alessandra Mongia, and Giampaolo Pompeo, and Julie di Bernardo, and Claudio Ceccarelli, and Giovanni Tallini, and Kerry J Rhoden
Medical Genetics Unit, Department of Medical and Surgical Sciences (DIMEC), University of Bologna, Bologna, Italy.

OBJECTIVE Iodide efflux from thyroid cells into the follicular lumen is essential for the synthesis of thyroid hormones, however, the pathways mediating this transport have only been partially identified. A calcium-activated pathway of iodide efflux has long been recognized, but its molecular identity unknown. Anoctamin 1 (ANO1) is a calcium-activated chloride channel (CaCC), and this study aims to investigate its contribution to iodide fluxes in thyroid cells. METHODS RT-PCR, immunohistochemistry, and live cell imaging with the fluorescent halide biosensor YFP-H148Q/I152L were used to study the expression, localization and function of ANO1 in thyroid cells. RESULTS ANO1 mRNA was detected in human thyroid tissue and FRTL-5 thyrocytes, and ANO1 protein was localized to the apical membrane of follicular cells. ATP induced a transient loss of iodide from FRTL-5 cells that was dependent on the mobilization of intracellular calcium, and was inhibited by CaCC/ANO1 inhibitors and siRNA against ANO1. Calcium-activated iodide efflux was also observed in CHO cells over-expressing the Sodium Iodide Symporter (NIS) and ANO1. CONCLUSIONS ANO1 in thyrocytes functions as a calcium-activated channel mediating iodide efflux, and may contribute to the rapid delivery of iodide into the follicular lumen for the synthesis of thyroid hormones following activation by calcium-mobilizing stimuli.

UI MeSH Term Description Entries
D007454 Iodides Inorganic binary compounds of iodine or the I- ion. Iodide
D002118 Calcium A basic element found in nearly all tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes. Coagulation Factor IV,Factor IV,Blood Coagulation Factor IV,Calcium-40,Calcium 40,Factor IV, Coagulation
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D000075369 Anoctamin-1 An anoctamin chloride channel expressed at high levels in the liver, skeletal muscle, and gastrointestinal muscles that functions in transepithelial anion transport and smooth muscle contraction. It is essential for the function of the INTERSTITIAL CELLS OF CAJAL and plays a major role in chloride conduction by airway epithelial cells and in tracheal cartilage development. TMEM16A Protein,Transmembrane Protein 16A,Anoctamin 1
D000255 Adenosine Triphosphate An adenine nucleotide containing three phosphate groups esterified to the sugar moiety. In addition to its crucial roles in metabolism adenosine triphosphate is a neurotransmitter. ATP,Adenosine Triphosphate, Calcium Salt,Adenosine Triphosphate, Chromium Salt,Adenosine Triphosphate, Magnesium Salt,Adenosine Triphosphate, Manganese Salt,Adenylpyrophosphate,CaATP,CrATP,Manganese Adenosine Triphosphate,MgATP,MnATP,ATP-MgCl2,Adenosine Triphosphate, Chromium Ammonium Salt,Adenosine Triphosphate, Magnesium Chloride,Atriphos,Chromium Adenosine Triphosphate,Cr(H2O)4 ATP,Magnesium Adenosine Triphosphate,Striadyne,ATP MgCl2
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D012333 RNA, Messenger RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm. Messenger RNA,Messenger RNA, Polyadenylated,Poly(A) Tail,Poly(A)+ RNA,Poly(A)+ mRNA,RNA, Messenger, Polyadenylated,RNA, Polyadenylated,mRNA,mRNA, Non-Polyadenylated,mRNA, Polyadenylated,Non-Polyadenylated mRNA,Poly(A) RNA,Polyadenylated mRNA,Non Polyadenylated mRNA,Polyadenylated Messenger RNA,Polyadenylated RNA,RNA, Polyadenylated Messenger,mRNA, Non Polyadenylated
D013961 Thyroid Gland A highly vascularized endocrine gland consisting of two lobes joined by a thin band of tissue with one lobe on each side of the TRACHEA. It secretes THYROID HORMONES from the follicular cells and CALCITONIN from the parafollicular cells thereby regulating METABOLISM and CALCIUM level in blood, respectively. Thyroid,Gland, Thyroid,Glands, Thyroid,Thyroid Glands,Thyroids
D017136 Ion Transport The movement of ions across energy-transducing cell membranes. Transport can be active, passive or facilitated. Ions may travel by themselves (uniport), or as a group of two or more ions in the same (symport) or opposite (antiport) directions. Antiport,Ion Cotransport,Ion Exchange, Intracellular,Symport,Uniport,Active Ion Transport,Facilitated Ion Transport,Passive Ion Transport,Cotransport, Ion,Exchange, Intracellular Ion,Intracellular Ion Exchange,Ion Transport, Active,Ion Transport, Facilitated,Ion Transport, Passive,Transport, Active Ion,Transport, Ion
D051381 Rats The common name for the genus Rattus. Rattus,Rats, Laboratory,Rats, Norway,Rattus norvegicus,Laboratory Rat,Laboratory Rats,Norway Rat,Norway Rats,Rat,Rat, Laboratory,Rat, Norway,norvegicus, Rattus

Related Publications

Carmela Iosco, and Cristina Cosentino, and Laura Sirna, and Roberta Romano, and Silvia Cursano, and Alessandra Mongia, and Giampaolo Pompeo, and Julie di Bernardo, and Claudio Ceccarelli, and Giovanni Tallini, and Kerry J Rhoden
October 2015, Current opinion in endocrinology, diabetes, and obesity,
Carmela Iosco, and Cristina Cosentino, and Laura Sirna, and Roberta Romano, and Silvia Cursano, and Alessandra Mongia, and Giampaolo Pompeo, and Julie di Bernardo, and Claudio Ceccarelli, and Giovanni Tallini, and Kerry J Rhoden
June 2014, Kidney international,
Carmela Iosco, and Cristina Cosentino, and Laura Sirna, and Roberta Romano, and Silvia Cursano, and Alessandra Mongia, and Giampaolo Pompeo, and Julie di Bernardo, and Claudio Ceccarelli, and Giovanni Tallini, and Kerry J Rhoden
December 1985, Biochimica et biophysica acta,
Carmela Iosco, and Cristina Cosentino, and Laura Sirna, and Roberta Romano, and Silvia Cursano, and Alessandra Mongia, and Giampaolo Pompeo, and Julie di Bernardo, and Claudio Ceccarelli, and Giovanni Tallini, and Kerry J Rhoden
July 2002, The Journal of clinical endocrinology and metabolism,
Carmela Iosco, and Cristina Cosentino, and Laura Sirna, and Roberta Romano, and Silvia Cursano, and Alessandra Mongia, and Giampaolo Pompeo, and Julie di Bernardo, and Claudio Ceccarelli, and Giovanni Tallini, and Kerry J Rhoden
October 2015, Pflugers Archiv : European journal of physiology,
Carmela Iosco, and Cristina Cosentino, and Laura Sirna, and Roberta Romano, and Silvia Cursano, and Alessandra Mongia, and Giampaolo Pompeo, and Julie di Bernardo, and Claudio Ceccarelli, and Giovanni Tallini, and Kerry J Rhoden
December 2023, American journal of physiology. Lung cellular and molecular physiology,
Carmela Iosco, and Cristina Cosentino, and Laura Sirna, and Roberta Romano, and Silvia Cursano, and Alessandra Mongia, and Giampaolo Pompeo, and Julie di Bernardo, and Claudio Ceccarelli, and Giovanni Tallini, and Kerry J Rhoden
November 2023, bioRxiv : the preprint server for biology,
Carmela Iosco, and Cristina Cosentino, and Laura Sirna, and Roberta Romano, and Silvia Cursano, and Alessandra Mongia, and Giampaolo Pompeo, and Julie di Bernardo, and Claudio Ceccarelli, and Giovanni Tallini, and Kerry J Rhoden
August 2014, Endocrinology,
Carmela Iosco, and Cristina Cosentino, and Laura Sirna, and Roberta Romano, and Silvia Cursano, and Alessandra Mongia, and Giampaolo Pompeo, and Julie di Bernardo, and Claudio Ceccarelli, and Giovanni Tallini, and Kerry J Rhoden
May 2014, Kidney international,
Carmela Iosco, and Cristina Cosentino, and Laura Sirna, and Roberta Romano, and Silvia Cursano, and Alessandra Mongia, and Giampaolo Pompeo, and Julie di Bernardo, and Claudio Ceccarelli, and Giovanni Tallini, and Kerry J Rhoden
January 2024, Handbook of experimental pharmacology,
Copied contents to your clipboard!