Biomaterial Database

Localization of Na(+)-K(+)-ATPase α/β, Na(+)-K(+)-2Cl-cotransporter 1 and aquaporin-5 in human eccrine sweat glands. 2014

Mingjun Zhang, and Shaopeng Zeng, and Lei Zhang, and Haihong Li, and Lu Chen, and Xiang Zhang, and Xuexue Li, and Changmin Lin, and Shenyou Shu, and Sitian Xie, and Yunpu He, and Xiaoyan Mao, and Lihong Peng, and Lungang Shi, and Lvjun Yang, and Shijie Tang, and Xiaobing Fu

Burn and Plastic Surgery Unit, The Second Affiliated Hospital, Shantou University Medical College, North DongXia Road, Shantou, Guangdong Province 515041, PR China.

In order to evaluate the function of the repaired or regenerated eccrine sweat glands, we must first localize the proteins involved in sweat secretion and absorption in normal human eccrine sweat glands. In our studies, the cellular localization of Na(+)-K(+)-ATPase α/β, Na(+)-K(+)-2Cl-cotransporter 1 (NKCC1) and aquaporin-5 (AQP5) in eccrine sweat glands were detected by immunoperoxidase labeling. The results showed that Na(+)-K(+)-ATPase α was immunolocalized in the cell membrane of the basal layer and suprabasal layer cells of the epidermis, the basolateral membrane of the secretory coils, and the cell membrane of the outer cells and the basolateral membrane of the luminal cells of the ducts. The localization of Na(+)-K(+)-ATPase β in the secretory coils was the same as Na(+)-K(+)-ATPase α, but Na(+)-K(+)-ATPase β labeling was absent in the straight ducts and epidermis. NKCC1 labeling was seen only in the basolateral membrane of the secretory coils. AQP5 was strongly localized in the apical membrane and weakly localized in the cytoplasm of secretory epithelial cells. The different distribution of these proteins in eccrine sweat glands was related to their functions in sweat secretion and absorption.

UI	MeSH Term	Description	Entries
D007150	Immunohistochemistry	Histochemical localization of immunoreactive substances using labeled antibodies as reagents.	Immunocytochemistry,Immunogold Techniques,Immunogold-Silver Techniques,Immunohistocytochemistry,Immunolabeling Techniques,Immunogold Technics,Immunogold-Silver Technics,Immunolabeling Technics,Immunogold Silver Technics,Immunogold Silver Techniques,Immunogold Technic,Immunogold Technique,Immunogold-Silver Technic,Immunogold-Silver Technique,Immunolabeling Technic,Immunolabeling Technique,Technic, Immunogold,Technic, Immunogold-Silver,Technic, Immunolabeling,Technics, Immunogold,Technics, Immunogold-Silver,Technics, Immunolabeling,Technique, Immunogold,Technique, Immunogold-Silver,Technique, Immunolabeling,Techniques, Immunogold,Techniques, Immunogold-Silver,Techniques, Immunolabeling
D008297	Male		Males
D004439	Eccrine Glands	Simple sweat glands that secrete sweat directly onto the SKIN.	Eccrine Gland,Gland, Eccrine,Glands, Eccrine
D005260	Female		Females
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000254	Sodium-Potassium-Exchanging ATPase	An enzyme that catalyzes the active transport system of sodium and potassium ions across the cell wall. Sodium and potassium ions are closely coupled with membrane ATPase which undergoes phosphorylation and dephosphorylation, thereby providing energy for transport of these ions against concentration gradients.	ATPase, Sodium, Potassium,Adenosinetriphosphatase, Sodium, Potassium,Na(+)-K(+)-Exchanging ATPase,Na(+)-K(+)-Transporting ATPase,Potassium Pump,Sodium Pump,Sodium, Potassium ATPase,Sodium, Potassium Adenosinetriphosphatase,Sodium-Potassium Pump,Adenosine Triphosphatase, Sodium, Potassium,Na(+) K(+)-Transporting ATPase,Sodium, Potassium Adenosine Triphosphatase,ATPase Sodium, Potassium,ATPase, Sodium-Potassium-Exchanging,Adenosinetriphosphatase Sodium, Potassium,Pump, Potassium,Pump, Sodium,Pump, Sodium-Potassium,Sodium Potassium Exchanging ATPase,Sodium Potassium Pump
D000328	Adult	A person having attained full growth or maturity. Adults are of 19 through 44 years of age. For a person between 19 and 24 years of age, YOUNG ADULT is available.	Adults
D051402	Aquaporin 5	Aquaporin 5 is a water-specific channel protein that is expressed primarily in alveolar, tracheal, and upper bronchial EPITHELIUM. It plays an important role in maintaining water HOMEOSTASIS in the LUNGS and may also regulate release of SALIVA and TEARS in the SALIVARY GLANDS and the LACRIMAL GLAND.	AQP5 Protein,Aquaporin 5 Protein,Aquaporin-5
D064506	Solute Carrier Family 12, Member 2	Na-K-Cl transporter ubiquitously expressed. It plays a key role in salt secretion in epithelial cells and cell volume regulation in nonepithelial cells.	BSC2 Protein,BSC2 Transport Protein,Bumetanide-Sensitive Cotransporter 2,Bumetanide-Sensitive Sodium Potassium Chloride Cotransporter 2,NKCC1 Cotransporter,NKCC1 Protein,NKCC1 Transport Protein,SLC12A2 Cotransporter,SLC12A2 Protein,SLC12A2 Transporter,Sodium-Potassium-Chloride Cotransporter 1 Protein,Solute Carrier Family 12 (Sodium Potassium Chloride Transporters), Member 2,Bumetanide Sensitive Cotransporter 2,Bumetanide Sensitive Sodium Potassium Chloride Cotransporter 2,Cotransporter 2, Bumetanide-Sensitive,Cotransporter, NKCC1,Cotransporter, SLC12A2,Protein, BSC2,Protein, BSC2 Transport,Protein, NKCC1,Protein, NKCC1 Transport,Protein, SLC12A2,Sodium Potassium Chloride Cotransporter 1 Protein,Transport Protein, BSC2,Transport Protein, NKCC1,Transporter, SLC12A2