Structural and mechanistic insights into the bacterial amyloid secretion channel CsgG. 2014

Parveen Goyal, and Petya V Krasteva, and Nani Van Gerven, and Francesca Gubellini, and Imke Van den Broeck, and Anastassia Troupiotis-Tsaïlaki, and Wim Jonckheere, and Gérard Péhau-Arnaudet, and Jerome S Pinkner, and Matthew R Chapman, and Scott J Hultgren, and Stefan Howorka, and Rémi Fronzes, and Han Remaut
1] Structural and Molecular Microbiology, Structural Biology Research Center, VIB, Pleinlaan 2, 1050 Brussels, Belgium [2] Structural Biology Brussels, Vrije Universiteit Brussel, Pleinlaan 2, 1050 Brussels, Belgium.

Curli are functional amyloid fibres that constitute the major protein component of the extracellular matrix in pellicle biofilms formed by Bacteroidetes and Proteobacteria (predominantly of the α and γ classes). They provide a fitness advantage in pathogenic strains and induce a strong pro-inflammatory response during bacteraemia. Curli formation requires a dedicated protein secretion machinery comprising the outer membrane lipoprotein CsgG and two soluble accessory proteins, CsgE and CsgF. Here we report the X-ray structure of Escherichia coli CsgG in a non-lipidated, soluble form as well as in its native membrane-extracted conformation. CsgG forms an oligomeric transport complex composed of nine anticodon-binding-domain-like units that give rise to a 36-stranded β-barrel that traverses the bilayer and is connected to a cage-like vestibule in the periplasm. The transmembrane and periplasmic domains are separated by a 0.9-nm channel constriction composed of three stacked concentric phenylalanine, asparagine and tyrosine rings that may guide the extended polypeptide substrate through the secretion pore. The specificity factor CsgE forms a nonameric adaptor that binds and closes off the periplasmic face of the secretion channel, creating a 24,000 Å(3) pre-constriction chamber. Our structural, functional and electrophysiological analyses imply that CsgG is an ungated, non-selective protein secretion channel that is expected to employ a diffusion-based, entropy-driven transport mechanism.

UI MeSH Term Description Entries
D008074 Lipoproteins Lipid-protein complexes involved in the transportation and metabolism of lipids in the body. They are spherical particles consisting of a hydrophobic core of TRIGLYCERIDES and CHOLESTEROL ESTERS surrounded by a layer of hydrophilic free CHOLESTEROL; PHOSPHOLIPIDS; and APOLIPOPROTEINS. Lipoproteins are classified by their varying buoyant density and sizes. Circulating Lipoproteins,Lipoprotein,Lipoproteins, Circulating
D008954 Models, Biological Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment. Biological Model,Biological Models,Model, Biological,Models, Biologic,Biologic Model,Biologic Models,Model, Biologic
D008958 Models, Molecular Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures. Molecular Models,Model, Molecular,Molecular Model
D011487 Protein Conformation The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain). Conformation, Protein,Conformations, Protein,Protein Conformations
D002462 Cell Membrane The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells. Plasma Membrane,Cytoplasmic Membrane,Cell Membranes,Cytoplasmic Membranes,Membrane, Cell,Membrane, Cytoplasmic,Membrane, Plasma,Membranes, Cell,Membranes, Cytoplasmic,Membranes, Plasma,Plasma Membranes
D004058 Diffusion The tendency of a gas or solute to pass from a point of higher pressure or concentration to a point of lower pressure or concentration and to distribute itself throughout the available space. Diffusion, especially FACILITATED DIFFUSION, is a major mechanism of BIOLOGICAL TRANSPORT. Diffusions
D004926 Escherichia coli A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc. Alkalescens-Dispar Group,Bacillus coli,Bacterium coli,Bacterium coli commune,Diffusely Adherent Escherichia coli,E coli,EAggEC,Enteroaggregative Escherichia coli,Enterococcus coli,Diffusely Adherent E. coli,Enteroaggregative E. coli,Enteroinvasive E. coli,Enteroinvasive Escherichia coli
D000682 Amyloid A fibrous protein complex that consists of proteins folded into a specific cross beta-pleated sheet structure. This fibrillar structure has been found as an alternative folding pattern for a variety of functional proteins. Deposits of amyloid in the form of AMYLOID PLAQUES are associated with a variety of degenerative diseases. The amyloid structure has also been found in a number of functional proteins that are unrelated to disease. Amyloid Fibril,Amyloid Fibrils,Amyloid Substance,Fibril, Amyloid,Fibrils, Amyloid,Substance, Amyloid
D018360 Crystallography, X-Ray The study of crystal structure using X-RAY DIFFRACTION techniques. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) X-Ray Crystallography,Crystallography, X Ray,Crystallography, Xray,X Ray Crystallography,Xray Crystallography,Crystallographies, X Ray,X Ray Crystallographies
D018441 Biofilms Encrustations formed from microbes (bacteria, algae, fungi, plankton, or protozoa) embedded in an EXTRACELLULAR POLYMERIC SUBSTANCE MATRIX that is secreted by the microbes. They occur on body surfaces such as teeth (DENTAL DEPOSITS); inanimate objects, and bodies of water. Biofilms are prevented from forming by treating surfaces with DENTIFRICES; DISINFECTANTS; ANTI-INFECTIVE AGENTS; and anti-fouling agents. Biofilm

Related Publications

Parveen Goyal, and Petya V Krasteva, and Nani Van Gerven, and Francesca Gubellini, and Imke Van den Broeck, and Anastassia Troupiotis-Tsaïlaki, and Wim Jonckheere, and Gérard Péhau-Arnaudet, and Jerome S Pinkner, and Matthew R Chapman, and Scott J Hultgren, and Stefan Howorka, and Rémi Fronzes, and Han Remaut
January 2015, Channels (Austin, Tex.),
Parveen Goyal, and Petya V Krasteva, and Nani Van Gerven, and Francesca Gubellini, and Imke Van den Broeck, and Anastassia Troupiotis-Tsaïlaki, and Wim Jonckheere, and Gérard Péhau-Arnaudet, and Jerome S Pinkner, and Matthew R Chapman, and Scott J Hultgren, and Stefan Howorka, and Rémi Fronzes, and Han Remaut
November 2015, Trends in microbiology,
Parveen Goyal, and Petya V Krasteva, and Nani Van Gerven, and Francesca Gubellini, and Imke Van den Broeck, and Anastassia Troupiotis-Tsaïlaki, and Wim Jonckheere, and Gérard Péhau-Arnaudet, and Jerome S Pinkner, and Matthew R Chapman, and Scott J Hultgren, and Stefan Howorka, and Rémi Fronzes, and Han Remaut
May 2020, Progress in biophysics and molecular biology,
Parveen Goyal, and Petya V Krasteva, and Nani Van Gerven, and Francesca Gubellini, and Imke Van den Broeck, and Anastassia Troupiotis-Tsaïlaki, and Wim Jonckheere, and Gérard Péhau-Arnaudet, and Jerome S Pinkner, and Matthew R Chapman, and Scott J Hultgren, and Stefan Howorka, and Rémi Fronzes, and Han Remaut
April 2006, Trends in biochemical sciences,
Parveen Goyal, and Petya V Krasteva, and Nani Van Gerven, and Francesca Gubellini, and Imke Van den Broeck, and Anastassia Troupiotis-Tsaïlaki, and Wim Jonckheere, and Gérard Péhau-Arnaudet, and Jerome S Pinkner, and Matthew R Chapman, and Scott J Hultgren, and Stefan Howorka, and Rémi Fronzes, and Han Remaut
September 2019, Journal of molecular biology,
Parveen Goyal, and Petya V Krasteva, and Nani Van Gerven, and Francesca Gubellini, and Imke Van den Broeck, and Anastassia Troupiotis-Tsaïlaki, and Wim Jonckheere, and Gérard Péhau-Arnaudet, and Jerome S Pinkner, and Matthew R Chapman, and Scott J Hultgren, and Stefan Howorka, and Rémi Fronzes, and Han Remaut
October 2017, Annual review of cell and developmental biology,
Parveen Goyal, and Petya V Krasteva, and Nani Van Gerven, and Francesca Gubellini, and Imke Van den Broeck, and Anastassia Troupiotis-Tsaïlaki, and Wim Jonckheere, and Gérard Péhau-Arnaudet, and Jerome S Pinkner, and Matthew R Chapman, and Scott J Hultgren, and Stefan Howorka, and Rémi Fronzes, and Han Remaut
February 2010, Current opinion in structural biology,
Parveen Goyal, and Petya V Krasteva, and Nani Van Gerven, and Francesca Gubellini, and Imke Van den Broeck, and Anastassia Troupiotis-Tsaïlaki, and Wim Jonckheere, and Gérard Péhau-Arnaudet, and Jerome S Pinkner, and Matthew R Chapman, and Scott J Hultgren, and Stefan Howorka, and Rémi Fronzes, and Han Remaut
December 2014, Proceedings of the National Academy of Sciences of the United States of America,
Parveen Goyal, and Petya V Krasteva, and Nani Van Gerven, and Francesca Gubellini, and Imke Van den Broeck, and Anastassia Troupiotis-Tsaïlaki, and Wim Jonckheere, and Gérard Péhau-Arnaudet, and Jerome S Pinkner, and Matthew R Chapman, and Scott J Hultgren, and Stefan Howorka, and Rémi Fronzes, and Han Remaut
November 2012, Molecular microbiology,
Parveen Goyal, and Petya V Krasteva, and Nani Van Gerven, and Francesca Gubellini, and Imke Van den Broeck, and Anastassia Troupiotis-Tsaïlaki, and Wim Jonckheere, and Gérard Péhau-Arnaudet, and Jerome S Pinkner, and Matthew R Chapman, and Scott J Hultgren, and Stefan Howorka, and Rémi Fronzes, and Han Remaut
February 2017, Nature structural & molecular biology,
Copied contents to your clipboard!