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Using two antisera against atriopeptin III (AP III) which had different characteristics in cross-reactivities with atrial natriuretic peptide (ANP) analogs, we have measured an immunoreactive ANP (ir-ANP) in the heart extracts of several species. ir-ANP in atrial extracts showed both high and low molecular weights. Serial dilutions of atrial extracts from chickens, turtles, frogs, and fish yielded competition curves which were parallel to the standard curve of AP III with antiserum No. 4. In comparison with two serial dilution curves of atrial extracts made using two antisera, No. 4 and No. 9, we suggest that the amino acid at position 12 of ir-ANP in the atrial extracts of chicken, turtle, frog, and fish is not isoleucine but is some other amino acid.
S H Kim, and
K W Cho, and
G Y Koh, and
K H Seul, and
J N So, and
H Ryu
March 1990,
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S H Kim, and
K W Cho, and
G Y Koh, and
K H Seul, and
J N So, and
H Ryu
May 1986,
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S H Kim, and
K W Cho, and
G Y Koh, and
K H Seul, and
J N So, and
H Ryu
January 1989,
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S H Kim, and
K W Cho, and
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K H Seul, and
J N So, and
H Ryu
October 1993,
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S H Kim, and
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S H Kim, and
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December 1989,
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S H Kim, and
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S H Kim, and
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S H Kim, and
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H Ryu
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S H Kim, and
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