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Experiments were designed to investigate the phenomenon of endothelium-dependent relaxation (EDR) to acetylcholine in two animal models of insulin dependent diabetes mellitus. Thoracic aortas obtained from streptozotocin diabetic rats and genetically diabetic biobreeding rats (BB rats) were used in this study. Concentration-effect curves to acetylcholine were carried out on aortic rings under isometric tension. Following the induction of diabetes with streptozotocin, half of the animals were treated with daily intermediate acting insulin and the other half maintained without insulin for a period of 12 weeks before the experiment. The diabetic BB rats were also maintained on insulin. The EDR to acetylcholine was not impaired in the aortas of streptozotocin diabetic rats (insulin treated as well as untreated) compared to nondiabetic controls. The scanning electron microscopic (SEM) appearances of the aortic endothelium did not differ among the three groups of animals. However, the EDR to acetylcholine was found to be impaired in the aortas of diabetic BB rats. (Maximum relaxation: 25.3 +/- 5.0% of the contraction to norepinephrine compared to 52.2 +/- 5.3% in controls.) The SEM appearances of the aortic endothelium in the diabetic BB rats were found to be abnormal with edema and loss of definition of cell margins compared to nondiabetic controls. The differences in EDR to acetylcholine seen between the two animal models of diabetes may be related to the different aetiologies of diabetes in the animals.
T Kappagoda, and
L Jayakody, and
R Rajotte, and
A B Thomson, and
M P Senaratne
March 2015,
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T Kappagoda, and
L Jayakody, and
R Rajotte, and
A B Thomson, and
M P Senaratne
May 1995,
Clinical science (London, England : 1979),
T Kappagoda, and
L Jayakody, and
R Rajotte, and
A B Thomson, and
M P Senaratne
September 1999,
Zhongguo yao li xue bao = Acta pharmacologica Sinica,
T Kappagoda, and
L Jayakody, and
R Rajotte, and
A B Thomson, and
M P Senaratne
August 2004,
Anaesthesia and intensive care,
T Kappagoda, and
L Jayakody, and
R Rajotte, and
A B Thomson, and
M P Senaratne
October 1995,
Research communications in molecular pathology and pharmacology,
T Kappagoda, and
L Jayakody, and
R Rajotte, and
A B Thomson, and
M P Senaratne
January 1991,
Heart and vessels,
T Kappagoda, and
L Jayakody, and
R Rajotte, and
A B Thomson, and
M P Senaratne
January 1995,
General pharmacology,
T Kappagoda, and
L Jayakody, and
R Rajotte, and
A B Thomson, and
M P Senaratne
December 1991,
The Journal of pharmacy and pharmacology,
T Kappagoda, and
L Jayakody, and
R Rajotte, and
A B Thomson, and
M P Senaratne
October 1991,
General and comparative endocrinology,
T Kappagoda, and
L Jayakody, and
R Rajotte, and
A B Thomson, and
M P Senaratne
May 1987,
Naunyn-Schmiedeberg's archives of pharmacology,
