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Freshly isolated murine epidermal Langerhans cells (LC) are weak stimulators of resting T cells. Upon culture their phenotype changes, their stimulatory activity increases significantly, and they come to resemble lymphoid dendritic cells. Resident murine LC, therefore, might represent a reservoir of immature dendritic cells. We have now used enzyme cytochemistry, a panel of some 80 monoclonal antibodies, and immunofluorescence microscopy or two-color flow cytometry, as well as transmission electron microscopy, to analyse the phenotype and morphology of human LC before and after 2-4 d of bulk epidermal cell culture. In addition, LC were enriched from bulk epidermal cell cultures, and their stimulatory capacity was tested in the allogeneic mixed leukocyte reaction and the oxidative mitogenesis assay. Cultured human LC resembled human lymphoid dendritic cells in morphology, phenotype, and function. Specifically, LC became non-adherent upon culture and developed sheet-like processes (so-called "veils"), decreased their surface ATP/ADP'ase activity, and lost nonspecific esterase activity. As in the mouse, surface expression of MHC class I and II antigens increased significantly, and FcII receptors were significantly reduced. Markers that are expressed by dendritic cells (like CD40) appeared on LC following culture. Cultured human LC were potent T-cell stimulators. Our findings support the view that resident human LC, like murine LC, represent immature precursors of lymphoid dendritic cells in skin-draining lymph nodes.
N Romani, and
A Lenz, and
H Glassel, and
H Stössel, and
U Stanzl, and
O Majdic, and
P Fritsch, and
G Schuler
August 2005,
Chinese medical journal,
N Romani, and
A Lenz, and
H Glassel, and
H Stössel, and
U Stanzl, and
O Majdic, and
P Fritsch, and
G Schuler
February 2004,
Immunology and cell biology,
N Romani, and
A Lenz, and
H Glassel, and
H Stössel, and
U Stanzl, and
O Majdic, and
P Fritsch, and
G Schuler
October 1991,
The British journal of dermatology,
N Romani, and
A Lenz, and
H Glassel, and
H Stössel, and
U Stanzl, and
O Majdic, and
P Fritsch, and
G Schuler
May 2016,
Developmental and comparative immunology,
N Romani, and
A Lenz, and
H Glassel, and
H Stössel, and
U Stanzl, and
O Majdic, and
P Fritsch, and
G Schuler
January 1995,
Advances in experimental medicine and biology,
N Romani, and
A Lenz, and
H Glassel, and
H Stössel, and
U Stanzl, and
O Majdic, and
P Fritsch, and
G Schuler
December 2010,
Trends in immunology,
N Romani, and
A Lenz, and
H Glassel, and
H Stössel, and
U Stanzl, and
O Majdic, and
P Fritsch, and
G Schuler
January 1990,
International reviews of immunology,
N Romani, and
A Lenz, and
H Glassel, and
H Stössel, and
U Stanzl, and
O Majdic, and
P Fritsch, and
G Schuler
December 1999,
Veterinary immunology and immunopathology,
N Romani, and
A Lenz, and
H Glassel, and
H Stössel, and
U Stanzl, and
O Majdic, and
P Fritsch, and
G Schuler
January 2004,
Immunobiology,
N Romani, and
A Lenz, and
H Glassel, and
H Stössel, and
U Stanzl, and
O Majdic, and
P Fritsch, and
G Schuler
January 1980,
Immunological reviews,
