BIOMAT Database Logo BIOMAT Database Logo

Improved cDNA cloning into bacteriophage lambda gt11. 1989

L J van Doorn, and A van Belkum, and T Kos
Department of Virology, TNO Primate Center, Rijswijk, The Netherlands.

UI MeSH Term Description Entries
D010582 Bacteriophage lambda A temperate inducible phage and type species of the genus lambda-like viruses, in the family SIPHOVIRIDAE. Its natural host is E. coli K12. Its VIRION contains linear double-stranded DNA with single-stranded 12-base 5' sticky ends. The DNA circularizes on infection. Coliphage lambda,Enterobacteria phage lambda,Phage lambda,lambda Phage
D003001 Cloning, Molecular The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells. Molecular Cloning
D004247 DNA A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine). DNA, Double-Stranded,Deoxyribonucleic Acid,ds-DNA,DNA, Double Stranded,Double-Stranded DNA,ds DNA
D004926 Escherichia coli A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc. Alkalescens-Dispar Group,Bacillus coli,Bacterium coli,Bacterium coli commune,Diffusely Adherent Escherichia coli,E coli,EAggEC,Enteroaggregative Escherichia coli,Enterococcus coli,Diffusely Adherent E. coli,Enteroaggregative E. coli,Enteroinvasive E. coli,Enteroinvasive Escherichia coli
D005822 Genetic Vectors DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition. Cloning Vectors,Shuttle Vectors,Vectors, Genetic,Cloning Vector,Genetic Vector,Shuttle Vector,Vector, Cloning,Vector, Genetic,Vector, Shuttle,Vectors, Cloning,Vectors, Shuttle
D001483 Base Sequence The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence. DNA Sequence,Nucleotide Sequence,RNA Sequence,DNA Sequences,Base Sequences,Nucleotide Sequences,RNA Sequences,Sequence, Base,Sequence, DNA,Sequence, Nucleotide,Sequence, RNA,Sequences, Base,Sequences, DNA,Sequences, Nucleotide,Sequences, RNA

Related Publications

L J van Doorn, and A van Belkum, and T Kos
Cloning cDNA into lambda gt10 and lambda gt11.
January 1987, Methods in enzymology,
L J van Doorn, and A van Belkum, and T Kos
Direct cloning of lambda gt11 cDNA inserts into a plasmid vector.
January 1994, Methods in molecular biology (Clifton, N.J.),
L J van Doorn, and A van Belkum, and T Kos
Purification of cloning quality lambda gt11 DNA.
October 1993, BioTechniques,
L J van Doorn, and A van Belkum, and T Kos
Sequencing of cloned DNA using bacteriophage lambda gt11 templates.
January 1989, BioTechniques,
L J van Doorn, and A van Belkum, and T Kos
Direct cloning of cDNA inserts from lambda gt11 phage DNA into a plasmid vector by a novel and simple method.
February 1990, Genetic analysis, techniques and applications,
L J van Doorn, and A van Belkum, and T Kos
Purification by ammonium sulfate precipitation of bacteriophage lambda gt11 DNA for restriction analysis of cloned cDNA inserts.
May 1988, Analytical biochemistry,
L J van Doorn, and A van Belkum, and T Kos
Bacteriophage lambda cloning system for the construction of directional cDNA libraries.
June 1987, Proceedings of the National Academy of Sciences of the United States of America,
L J van Doorn, and A van Belkum, and T Kos
A lambda gt11 cDNA recombinant that encodes Dirofilaria immitis paramyosin.
June 1989, Molecular and biochemical parasitology,
L J van Doorn, and A van Belkum, and T Kos
Enzymatic amplification of specific cDNA inserts from lambda gt11 libraries.
September 1988, Nucleic acids research,
L J van Doorn, and A van Belkum, and T Kos
Bacteriophage lambda cloning vectors.
January 1988, Biotechnology (Reading, Mass.),
Copied contents to your clipboard!