BIOMAT Database Logo BIOMAT Database Logo

Isolation of cDNA clones encoding small nuclear ribonucleoparticle-associated proteins with different tissue specificities. 1989

S Li, and E S Klein, and A F Russo, and D M Simmons, and M G Rosenfeld
Eukaryotic Regulatory Biology Program, School of Medicine, University of California, San Diego, La Jolla 92093.

Alternative RNA processing, such as brain- and heart-specific generation of calcitonin gene-related peptide (CGRP) transcripts from the calcitonin/CGRP gene, is thought to be mediated by tissue-specific factors. We have cloned three related but distinct cDNAs encoding small nuclear ribonucleoparticle (snRNP)-associated proteins from rat PC12 cells. One clone (Sm51) has the capacity to encode a 240-amino acid protein and its RNA transcript is expressed selectively in rat brain and pituitary but not in heart. A related cDNA, designated Sm11, predicts a protein highly homologous to but distinct from Sm51. The Sm11 transcript is very abundant in heart but barely detectable in brain. Sm51 and Sm11 appear to encode the brain and heart forms of a 28-kDa snRNP-associated protein detected by anti-Sm serum, respectively. A third clone (Sm21) encodes a protein with an altered N terminus relative to Sm51. The Sm51 transcript is expressed in the pituitary, and analysis of the pituitaries of transgenic mice harboring a mouse metallothionein I promoter-calcitonin/CGRP fusion gene reveals the splice choice to be predominantly CGRP. In situ hybridization indicates Sm51 RNA is expressed throughout neuronal structures within rat brain, including the inferior colliculus, which does not possess the machinery to generate CGRP. Although Sm51 alone cannot be sufficient to account for CGRP splicing choice in all tissues, the demonstration of discrete tissue-specific expression patterns of closely related snRNP-associated proteins is consistent with their potential role in differential RNA processing events.

UI MeSH Term Description Entries
D008297 Male Males
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D009693 Nucleic Acid Hybridization Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503) Genomic Hybridization,Acid Hybridization, Nucleic,Acid Hybridizations, Nucleic,Genomic Hybridizations,Hybridization, Genomic,Hybridization, Nucleic Acid,Hybridizations, Genomic,Hybridizations, Nucleic Acid,Nucleic Acid Hybridizations
D009928 Organ Specificity Characteristic restricted to a particular organ of the body, such as a cell type, metabolic response or expression of a particular protein or antigen. Tissue Specificity,Organ Specificities,Specificities, Organ,Specificities, Tissue,Specificity, Organ,Specificity, Tissue,Tissue Specificities
D010673 Pheochromocytoma A usually benign, well-encapsulated, lobular, vascular tumor of chromaffin tissue of the ADRENAL MEDULLA or sympathetic paraganglia. The cardinal symptom, reflecting the increased secretion of EPINEPHRINE and NOREPINEPHRINE, is HYPERTENSION, which may be persistent or intermittent. During severe attacks, there may be HEADACHE; SWEATING, palpitation, apprehension, TREMOR; PALLOR or FLUSHING of the face, NAUSEA and VOMITING, pain in the CHEST and ABDOMEN, and paresthesias of the extremities. The incidence of malignancy is as low as 5% but the pathologic distinction between benign and malignant pheochromocytomas is not clear. (Dorland, 27th ed; DeVita Jr et al., Cancer: Principles & Practice of Oncology, 3d ed, p1298) Pheochromocytoma, Extra-Adrenal,Extra-Adrenal Pheochromocytoma,Extra-Adrenal Pheochromocytomas,Pheochromocytoma, Extra Adrenal,Pheochromocytomas,Pheochromocytomas, Extra-Adrenal
D011919 Rats, Inbred Strains Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations or by parent x offspring matings carried out with certain restrictions. This also includes animals with a long history of closed colony breeding. August Rats,Inbred Rat Strains,Inbred Strain of Rat,Inbred Strain of Rats,Inbred Strains of Rats,Rat, Inbred Strain,August Rat,Inbred Rat Strain,Inbred Strain Rat,Inbred Strain Rats,Inbred Strains Rat,Inbred Strains Rats,Rat Inbred Strain,Rat Inbred Strains,Rat Strain, Inbred,Rat Strains, Inbred,Rat, August,Rat, Inbred Strains,Rats Inbred Strain,Rats Inbred Strains,Rats, August,Rats, Inbred Strain,Strain Rat, Inbred,Strain Rats, Inbred,Strain, Inbred Rat,Strains, Inbred Rat
D001921 Brain The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of three major parts including PROSENCEPHALON (the forebrain); MESENCEPHALON (the midbrain); and RHOMBENCEPHALON (the hindbrain). The developed brain consists of CEREBRUM; CEREBELLUM; and other structures in the BRAIN STEM. Encephalon
D002116 Calcitonin A peptide hormone that lowers calcium concentration in the blood. In humans, it is released by thyroid cells and acts to decrease the formation and absorptive activity of osteoclasts. Its role in regulating plasma calcium is much greater in children and in certain diseases than in normal adults. Thyrocalcitonin,Calcitonin(1-32),Calcitrin,Ciba 47175-BA,Eel Calcitonin,Calcitonin, Eel,Ciba 47175 BA,Ciba 47175BA
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D003001 Cloning, Molecular The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells. Molecular Cloning

Related Publications

S Li, and E S Klein, and A F Russo, and D M Simmons, and M G Rosenfeld
Isolation of cDNA clones encoding T cell-specific membrane-associated proteins.
January 1984, Nature,
S Li, and E S Klein, and A F Russo, and D M Simmons, and M G Rosenfeld
Isolation of cDNA clones encoding T cell-specific membrane-associated proteins. 1984.
September 2005, Journal of immunology (Baltimore, Md. : 1950),
S Li, and E S Klein, and A F Russo, and D M Simmons, and M G Rosenfeld
Isolation and characterisation of wheat cDNA clones encoding PR4 proteins.
January 1999, DNA sequence : the journal of DNA sequencing and mapping,
S Li, and E S Klein, and A F Russo, and D M Simmons, and M G Rosenfeld
CRF2-4: isolation of cDNA clones encoding the human and mouse proteins.
February 1997, Gene,
S Li, and E S Klein, and A F Russo, and D M Simmons, and M G Rosenfeld
Isolation and characterization of cDNA clones encoding aldose reductase.
October 1989, Current eye research,
S Li, and E S Klein, and A F Russo, and D M Simmons, and M G Rosenfeld
Isolation and characterization of cDNA clones encoding jacalin isolectins.
March 1993, The Journal of biological chemistry,
S Li, and E S Klein, and A F Russo, and D M Simmons, and M G Rosenfeld
Isolation of cDNA clones encoding human histone macroH2A1 subtypes.
July 1998, Biochimica et biophysica acta,
S Li, and E S Klein, and A F Russo, and D M Simmons, and M G Rosenfeld
Isolation of cDNA clones encoding HLA-DR alpha chains.
November 1982, Proceedings of the National Academy of Sciences of the United States of America,
S Li, and E S Klein, and A F Russo, and D M Simmons, and M G Rosenfeld
Isolation of cDNA clones encoding proteins of complex structures: analysis of the Trypanosoma brucei cytoskeleton.
January 1992, Gene,
S Li, and E S Klein, and A F Russo, and D M Simmons, and M G Rosenfeld
Isolation and characterization of cDNA clones encoding antifreeze proteins of the pyrochroid beetle Dendroides canadensis.
March 2000, Journal of insect physiology,
Copied contents to your clipboard!